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Generation and characterization of monoclonal antibodies against choline kinase α and their potential use as diagnostic tools in cancer

AuthorsGallego Ortega, David ; Ramírez de Molina, Ana; Gutierrez, Ruth; Ramos, Maria Angeles ; Sarmentero Estrada, Jacinto; Cejas, Paloma; Nistal, Manuel; González Barón, Manuel; Lacal, Juan Carlos
KeywordsCholine kinase
Monoclonal antibodies
Cancer diagnosis
Tumor marker
Issue DateAug-2006
PublisherSpandidos Publications
CitationInternational Journal of Oncology 29(2): 335-340 (2006)
AbstractCholine kinase α (ChoKα) is a metabolic enzyme involved in the synthesis of phosphatidylcholine, recently implicated in cancer onset since it is overexpressed in a variety of human cancers such as mammary, lung, colorectal and prostate adenocarcinomas. Furthermore, overexpression of ChoKα in human HEK293T cells confers them oncogenic properties with the induction of tumors after subcutaneous injection in nude mice. ChoKα levels in tumor samples have been analyzed using polyclonal antibodies and Western blotting. These techniques have considerable limitations and do not allow for a precise and efficient evaluation of the real significance of ChoK overexpression in human carcinogenesis. We developed a set of monoclonal antibodies with high specificity and sensitivity against ChoKα, and characterized their properties. We provide evidence that the newly generated MoAbs against ChoKα have potential use in cancer diagnosis by conventional immunohistochemistry techniques.
Description6 pages, 3 figures, 1 table.
Publisher version (URL)http://www.spandidos-publications.com/ijo/article.jsp?article_id=ijo_29_2_335
Appears in Collections:(IIBM) Artículos
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