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http://hdl.handle.net/10261/24731
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Martín-Villar, Ester | - |
dc.contributor.author | Megías, Diego | - |
dc.contributor.author | Castel, Susanna | - |
dc.contributor.author | Yurrita, María M. | - |
dc.contributor.author | Vilaró, Senén | - |
dc.contributor.author | Quintanilla, Miguel | - |
dc.date.accessioned | 2010-05-26T12:35:51Z | - |
dc.date.available | 2010-05-26T12:35:51Z | - |
dc.date.issued | 2006-11-01 | - |
dc.identifier.citation | Journal of Cell Science 119(21): 4541-4553 | en_US |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.uri | http://hdl.handle.net/10261/24731 | - |
dc.description | 13 pages, 8 figures, 1 table. | en_US |
dc.description.abstract | Podoplanin is a small membrane mucin expressed in tumors associated with malignant progression. It is enriched at cell-surface protrusions where it colocalizes with members of the ERM (ezrin, radixin, moesin) protein family. Here, we found that human podoplanin directly interacts with ezrin (and moesin) in vitro and in vivo through a cluster of basic amino acids within its cytoplasmic tail, mainly through a juxtamembrane dipeptide RK. Podoplanin induced an epithelial-mesenchymal transition in MDCK cells linked to the activation of RhoA and increased cell migration and invasiveness. Fluorescence time-lapse video observations in migrating cells indicate that podoplanin might be involved in ruffling activity as well as in retractive processes. By using mutant podoplanin constructs fused to green fluorescent protein we show that association of the cytoplasmic tail with ERM proteins is required for upregulation of RhoA activity and epithelial-mesenchymal transition. Furthermore, expression of either a dominant-negative truncated variant of ezrin or a dominant-negative mutant form of RhoA blocked podoplanin-induced RhoA activation and epithelial-mesenchymal transition. These results provide a mechanistic basis to understand the role of podoplanin in cell migration or invasiveness. | en_US |
dc.description.sponsorship | This work was supported by grants: SAF2004-04902 from the Ministry of Education and Science (MEC), GR/SAL/0871/2004 from the Autonomous Community of Madrid (CAM) and RTICCC CO3/10 from the ‘Instituto de Salud Carlos III’ (FIS) of Spain. E.M.-V. and M.M.Y. were the recipients of a postgraduate I3P fellowship from the Spanish Research Council (CSIC) and an MEC predoctoral fellowship, respectively. | en_US |
dc.format.extent | 6081921 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Company of Biologists | en_US |
dc.rights | openAccess | en_US |
dc.subject | Podoplanin/PA2.26 antigen | en_US |
dc.subject | Ezrin | en_US |
dc.subject | RhoA | en_US |
dc.subject | Epithelial-mesenchymal transition | en_US |
dc.subject | Cell migration | en_US |
dc.title | Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1242/jcs.03218 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1242/jcs.03218 | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (IIBM) Artículos |
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ERM proteins.pdf | 5,94 MB | Adobe PDF | Visualizar/Abrir |
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