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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24731
Título

Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition

AutorMartín-Villar, Ester ; Megías, Diego; Castel, Susanna; Yurrita, María M. ; Vilaró, Senén; Quintanilla, Miguel
Palabras clavePodoplanin/PA2.26 antigen
Ezrin
RhoA
Epithelial-mesenchymal transition
Cell migration
Fecha de publicación1-nov-2006
EditorCompany of Biologists
CitaciónJournal of Cell Science 119(21): 4541-4553
ResumenPodoplanin is a small membrane mucin expressed in tumors associated with malignant progression. It is enriched at cell-surface protrusions where it colocalizes with members of the ERM (ezrin, radixin, moesin) protein family. Here, we found that human podoplanin directly interacts with ezrin (and moesin) in vitro and in vivo through a cluster of basic amino acids within its cytoplasmic tail, mainly through a juxtamembrane dipeptide RK. Podoplanin induced an epithelial-mesenchymal transition in MDCK cells linked to the activation of RhoA and increased cell migration and invasiveness. Fluorescence time-lapse video observations in migrating cells indicate that podoplanin might be involved in ruffling activity as well as in retractive processes. By using mutant podoplanin constructs fused to green fluorescent protein we show that association of the cytoplasmic tail with ERM proteins is required for upregulation of RhoA activity and epithelial-mesenchymal transition. Furthermore, expression of either a dominant-negative truncated variant of ezrin or a dominant-negative mutant form of RhoA blocked podoplanin-induced RhoA activation and epithelial-mesenchymal transition. These results provide a mechanistic basis to understand the role of podoplanin in cell migration or invasiveness.
Descripción13 pages, 8 figures, 1 table.
Versión del editorhttp://dx.doi.org/10.1242/jcs.03218
URIhttp://hdl.handle.net/10261/24731
DOI10.1242/jcs.03218
ISSN0021-9533
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