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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24675

Spironolactone and its main metabolite, canrenoic acid, block human ether-a-go-go–related gene channels

AutorCaballero, Ricardo; Moreno de Alborán, Ignacio; González, Teresa ; Arias, Cristina; Valenzuela, Carmen ; Delpón, Eva; Tamargo, Juan
Palabras claveIon channels
Potassium channels
Patch-clamp techniques
Fecha de publicación18-feb-2003
EditorAmerican Heart Association
CitaciónCirculation 107(6): 889-895 (2003)
ResumenBackground— It has been demonstrated that spironolactone (SP) decreases the QT dispersion in chronic heart failure. In this study, the effects of SP and its metabolite, canrenoic acid (CA), on human ether-a-go-go–related gene (HERG) currents were analyzed. Methods and Results— HERG currents elicited in stably transfected Chinese hamster ovary cells were measured with the whole-cell patch-clamp technique. SP decreased HERG currents in a concentration-dependent manner (IC50=23.0±1.5 µmol/L) and shifted the midpoint of the activation curve to more negative potentials (Vh=-13.1±3.4 versus -18.9±3.6 mV, P<0.05) without modifying the activation and deactivation kinetics. SP-induced block (1 µmol/L) appeared at the range of membrane potentials coinciding with that of channel activation, and thereafter, it remained constant, reaching 24.7±3.8% at +60 mV (n=6, P<0.05). CA (0.01 nmol/L to 500 µmol/L) blocked HERG channels in a voltage- and frequency-independent manner. CA at 1 nmol/L shifted the midpoint of the activation curve to -19.9±1.8 mV and accelerated the time course of channel activation ({tau}=1064±125 versus 820±93 ms, n=11, P<0.01). The envelope of the tail test demonstrated that at the very beginning of the pulses to +40 mV (25 ms), a certain amount of block was apparent (31.3±9.9%). CA did not modify the voltage-dependence of HERG channel inactivation (Vh=-60.8±5.6 versus -62.9±3.1 mV, n=6, P>0.05) or the kinetics of the reactivation process at any potential tested. CA and aldosterone also blocked the native IKr in guinea-pig ventricular myocytes.
Descripción8 pages, 6 figures.
Versión del editorhttp://dx.doi.org/10.1161/01.CIR.0000048189.58449.F7
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