English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/246695
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening

AuthorsSentandreu, Enrique CSIC ORCID; Fuente-García, Claudia; Pardo, Olga; Oliván, Mamen; León, Núria; Aldai, Noelia CSIC ORCID; Yusà, Vicent; Sentandreu, Miguel Ángel
KeywordsHigh-resolution proteomics
Meat biomarkers
Meat quality assessment
Rapid proteomic screening
Pre-slaughter stress
Issue Date25-Jun-2021
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 69 (26): 7478-7487 (2021)
AbstractAn understanding of biological mechanisms that could be involved in the stress response of animal cattle prior to slaughter is critical to create effective strategies aiming at the production of high-quality meat. The sarcoplasmic proteome of directly extracted samples from normal and high ultimate pH (pHu) meat groups was studied through a straightforward gel-free strategy supported by liquid chromatography hybrid quadrupole-Orbitrap high-resolution mass spectrometry (LC-HRMS) analysis. A stepped proteomic pipeline combining rapid biomarker hunting supported by qualitative protein Mascot scores followed by targeted label-free peptide quantification revealed 26 descriptors that characterized meat groups assayed. The functional study of the proposed biomarkers suggested their relevant role in metabolic, chaperone/stress-related, muscle contractility/fiber organization, and transport activities. The efficiency, flexibility, rapidity, and easiness of the methodology proposed can positively contribute to the creation of innovative proteomic alternatives addressing meat quality assessment.
Publisher version (URL)https://doi.org/10.1021/acs.jafc.1c02016
URIhttp://hdl.handle.net/10261/246695
DOIhttp://dx.doi.org/10.1021/acs.jafc.1c02016
ISSN0021-8561
Appears in Collections:(IATA) Artículos
Files in This Item:
File Description SizeFormat 
JAFC2021-Sentandreu.pdfArtículo principal1,64 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.