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Title

Identification of C-terminal motifs responsible for transmission of inhibition by ATP of mammalian phosphofructokinase, and their contribution to other allosteric effects

AuthorsMartínez-Costa, Oscar H. ; Hermida, Carmen; Sánchez-Martínez, Cristina; Santamaría, Belén; Aragón, Juan J.
KeywordsAllosteric regulation
Allosteric transition
Enzyme engineering
Enzyme regulation
Glycolysis
Phosphofructokinase
Issue Date1-Jan-2004
PublisherPortland Press
CitationBiochemical Journal 377(1): 77-84 (2004)
AbstractSystematic deletions and point mutations in the C-terminal extension of mammalian PFK (phosphofructokinase) led us to identify Leu-767 and Glu-768 of the M-type isoform (PFK-M) as the motifs responsible for the role of this region in inhibition by MgATP. These amino acids are the only residues of the C-terminus that are conserved in all mammalian isoforms, and were found to have a similar function in the C-type isoenzyme. Both residues in PFK-C and Leu-767 in PFK-M were also observed to be critical for inhibition by citrate, which is synergistic with that by MgATP. Binding studies utilizing titration of intrinsic protein fluorescence indicated that the C-terminal part of the enzyme participates in the signal transduction route from the MgATP inhibitory site to the catalytic site, but does not contribute to the binding of this inhibitor, whereas it is essential for the binding of citrate. Mutations of the identified structural motifs did not alter either the action of other allosteric effectors that also interact with MgATP, such as the inhibitor phosphoenolpyruvate and the strong activator fructose 2,6-bisphosphate, or the co-operative effect of fructose 6-phosphate. The latter data provide evidence that activation by fructose 2,6-bisphosphate and fructose 6-phosphate co-operativity are not linked to the same allosteric transition as that mediating inhibition by MgATP.
Description8 pages, 7 figures, 1 table.
Publisher version (URL)http://www.biochemj.org/bj/377/0077/bj3770077.htm
URIhttp://hdl.handle.net/10261/24645
ISSN0264-6021
Appears in Collections:(IIBM) Artículos
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