English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24644
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 42 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

A permissive retinoid X receptor/thyroid hormone receptor heterodimer allows stimulation of prolactin gene transcription by thyroid hormone and 9-cis-retinoic acid

AutorCastillo, Ana I. ; Sánchez-Martínez, Ruth; Moreno, José L.; Martínez-Iglesias, Olaia ; Palacios, Daniela; Aranda, Ana
Fecha de publicaciónene-2004
EditorAmerican Society for Microbiology
CitaciónMolecular and Cellular Biology 24(2): 502-513 (2004)
ResumenHeterodimers of the retinoid X receptor (RXR) with the thyroid hormone receptor (TR) are considered to be nonpermissive. It is believed that within these complexes RXR acts as a "silent partner." We demonstrate here that a permissive heterodimer mediates stimulation of prolactin expression by the thyroid hormone T3 and by 9-cis retinoic acid (9-cis-RA). A response element located in the prolactin distal enhancer mediates transactivation by both ligands in pituitary cells, and RXR recruits coactivators when bound to this element as a heterodimer with TR. Furthermore, transcription by the RXR agonist can be obtained in CV-1 cells only after overexpression of coactivators, and overexpression of corepressors inhibits the response in pituitary cells. Thus, cell type-specific differences in coregulator recruitment can determine the cellular response to both ligands. Coactivator recruitment by 9-cis-RA requires the ligand-dependent transactivation domains (AF-2) of both heterodimeric partners. Interestingly, the presence of the RXR ligand can overcome the deleterious effect of the AF-2 mutation E401Q on association with coactivators and transactivation. These results demonstrate an unexpected role for RXR in TR signaling and show that in particular cellular environments this receptor can act as a "nonsilent" partner of TR, allowing stimulation by RXR agonists.
Descripción12 pages, 10 figures.
Versión del editorhttp://dx.doi.org/10.1128/MCB.24.2.502-513.2004
URIhttp://hdl.handle.net/10261/24644
DOI10.1128/MCB.24.2.502-513.2004
ISSN0270-7306
Aparece en las colecciones: (IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.