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Título

Trehalose accumulation induced during the oxidative stress response is independent of TPS1 mRNA levels in Candida albicans

AutorZaragoza, Oscar; González-Párraga, Pilar; Pedreño, Yolanda; Alvarez-Peral, Francisco J.; Argüelles, Juan-Carlos
Palabras claveCandida albicans
Tps1 gene
Mrna levels
Trehalose
Oxidative stress
Fecha de publicación6-jun-2003
EditorViguera Editores
CitaciónInternational Microbiology 6(2): 121-125 (2003)
ResumenGrowing cells of the Candida albicans trehalose- deficient mutant tps1/tps1 were extremely sensitive to severe oxidative stress exposure (H2O2). However, their viability was not affected after saline stress or heatshock treatments, being roughly equivalent to that of the parental strain. In wild-type cells, these adverse conditions induced the intracellular accumulation of trehalose together with activation of trehalose-6P synthase, whereas the endogenous trehalose content and the corresponding biosynthetic activity were barely detectable in the tps1/tps1 mutant. The addition of cycloheximide did not prevent the marked induction of trehalose-6P synthase activity. Furthermore, the presence of H2O2 decreased the level of TPS1 mRNA expression. Hence, the conspicuous trehalose accumulation in response to oxidative stress is not induced by increased transcription of TPS1. Our results are consistent with a specific requirement of trehalose in order to withstand a severe oxidative stress in C. albicans, and suggest that trehalose accumulation observed under these conditions is a complex process that most probably involves post-translational modifications of the trehalose synthase complex.
Descripción5 pages, 2 figures, 2 tables.
Versión del editorhttp://dx.doi.org/10.1007/s10123-003-0119-y
URIhttp://hdl.handle.net/10261/24596
DOI10.1007/s10123-003-0119-y
ISSN1139-6709
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