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Título: | VRK1 and AURKB form a complex that cross inhibit their kinase activity and the phosphorylation of histone H3 in the progression of mitosis |
Autor: | Moura, David S. CSIC ORCID; Campillo-Marcos, Ignacio CSIC ORCID; Vázquez-Cedeira, Marta CSIC; Lazo, Pedro A. CSIC ORCID | Palabras clave: | VRK1 Aurora kinase B Survivin Histone H3 Kinase Phosphorylation |
Fecha de publicación: | 2018 | Editor: | Springer Nature | Citación: | Cellular and Molecular Life Sciences 75: 2591-2611 (2018) | Resumen: | Regulation of cell division requires the integration of signals implicated in chromatin reorganization and coordination of its sequential changes in mitosis. Vaccinia-related kinase 1 (VRK1) and Aurora B (AURKB) are two nuclear kinases involved in different steps of cell division. We have studied whether there is any functional connection between these two nuclear kinases, which phosphorylate histone H3 in Thr3 and Ser10, respectively. VRK1 and AURKB are able to form a stable protein complex, which represents only a minor subpopulation of each kinase within the cell and is detected following nocodazole release. Each kinase is able to inhibit the kinase activity of the other kinase, as well as inhibit their specific phosphorylation of histone H3. In locations where the two kinases interact, there is a different pattern of histone modifications, indicating that there is a local difference in chromatin during mitosis because of the local complexes formed by these kinases and their asymmetric intracellular distribution. Depletion of VRK1 downregulates the gene expression of BIRC5 (survivin) that recognizes H3-T3ph, both are dependent on the activity of VRK1, and is recovered with kinase active murine VRK1, but not with a kinase-dead protein. The H3–Thr3ph–survivin complex is required for AURB recruitment, and their loss prevents the localization of ACA and AURKB in centromeres. The cross inhibition of the kinases at the end of mitosis might facilitate the formation of daughter cells. A sequential role for VRK1, AURKB, and haspin in the progression of mitosis is proposed. | Descripción: | © The Author(s) 2018. | Versión del editor: | http://dx.doi.org/10.1007/s00018-018-2746-7 | URI: | http://hdl.handle.net/10261/245421 | DOI: | 10.1007/s00018-018-2746-7 | ISSN: | 1420-682X | E-ISSN: | 1420-9071 |
Aparece en las colecciones: | (IBMCC) Artículos |
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VRK1_Moura_PV_Art2018.pdf | 5,02 MB | Unknown | Visualizar/Abrir |
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