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Título

Growth factor regulation of uncoupling protein-1 mRNA expression in brown adipocytes

AutorGarcía, Bibian; Obregón, María Jesús
Palabras claveProliferation
Differentiation
Thermogenesis
Fecha de publicaciónene-2002
EditorAmerican Physiological Society
CitaciónAmerican Journal of Physiology - Cell Physiology 282(1): C105-C112 (2002)
ResumenTo study the effect of the mitogens epidermal growth factor (EGF), acidic and basic fibroblast growth factors (aFGF and bFGF), and vasopressin on brown adipocyte differentiation, we analyzed the expression of uncoupling protein-1 (UCP-1) mRNA. Quiescent brown preadipocytes express high levels of UCP-1 mRNA in response to triiodothyronine (T3) and norepinephrine (NE). The addition of serum or the mitogenic condition aFGF + vasopressin + NE or EGF + vasopressin + NE decreases UCP-1 mRNA. A second addition of mitogens further decreases UCP-1 mRNA. Treatment with aFGF or bFGF alone increases UCP-1 mRNA, whereas the addition of EGF or vasopressin dramatically reduces UCP-1 mRNA levels. The continuous presence of T3 increases UCP-1 mRNA levels in cells treated with EGF, aFGF, or bFGF. The effect of T3 on the stimulation of DNA synthesis also was tested. T3 inhibits the mitogenic activity of aFGF and bFGF. In conclusion, mitogens like aFGF or bFGF allow brown adipocyte differentiation, whereas EGF and vasopressin inhibit the differentiation process. T3 behaves as an important hormone that regulates both brown adipocyte proliferation and differentiation.
Descripción9 pages, 7 figures.
Versión del editorhttp://dx.doi.org/10.1152/ajpcell.01396.2000
URIhttp://hdl.handle.net/10261/24535
DOI10.1152/ajpcell.01396.2000
ISSN0363-6143
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