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dc.contributor.authorMartínez de Mena, Raquel-
dc.contributor.authorHernández, Arturo-
dc.contributor.authorObregón, María Jesús-
dc.date.accessioned2010-05-19T13:00:11Z-
dc.date.available2010-05-19T13:00:11Z-
dc.date.issued2002-05-
dc.identifier.citationAJP - Endocrinology and Metabolism 282(5): E1119-E1127 (2002)en_US
dc.identifier.issn0193-1849-
dc.identifier.urihttp://hdl.handle.net/10261/24502-
dc.description10 pages, 6 figures.en_US
dc.description.abstractType II 5'-iodothyronine deiodinase (D2), produces triiodothyronine (T(3)) and is stimulated by cold exposure via norepinephrine (NE) release in brown adipose tissue. Cultured rat brown adipocytes require T(3) for the adrenergic stimulation of D2 activity. D2 mRNA expression in cultured brown adipocytes is undetectable with the use of basal conditions or NE without T(3). Full D2 expression is achieved using NE + T(3), especially after prolonged T(3) exposure. beta(3)-Adrenergic agonists mimic the NE action, whereas cAMP analogs do not. Prolonged exposure to T(3) alone increases D2 mRNA. High T(3) doses (500 nM) inhibit the adrenergic stimulation of D2 activity while increasing D2 mRNA. The effects obtained with NE + T(3) or T(3) alone are suppressed by actinomycin, but not by cycloheximide, which leads to accumulation of short D2 mRNA transcripts. Prolonged or short exposure to T(3) did not change D2 mRNA half-life, but T(3) seemed to elongate it. In conclusion, T(3) is an absolute requirement for the adrenergic stimulation of D2 mRNA in brown adipocytes. T(3) upregulates D2 mRNA, an effect that might involve stimulation of factors required for transcription or for stabilization of D2 mRNA.en_US
dc.description.sponsorshipThis work was supported by research grants PB 95–0097 from Dirección General de Investigación Científica y Técnica, FISS 94/0274 and 99/0813 from Fondo de Investigaciones Sanitarias (FIS), and CAM 08.6/0030/1998 from Comunidad de Madrid (CAM) (Spain). R. Martínez de Mena was supported by research grants FISS 94/0274 (predoctoral studies) and CAM 08.6/0030/1998 (as postdoctoral).en_US
dc.format.extent333718 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherAmerican Physiological Societyen_US
dc.rightsclosedAccessen_US
dc.subjectNorepinephrineen_US
dc.subjectUncoupling protein-1en_US
dc.titleTriiodothyronine is required for the stimulation of type II 5'-deiodinase mRNA in rat brown adipocytesen_US
dc.typeartículoen_US
dc.identifier.doi10.1152/ajpendo.00433.2001-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1152/ajpendo.00433.2001en_US
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
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