English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24502
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 23 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Triiodothyronine is required for the stimulation of type II 5'-deiodinase mRNA in rat brown adipocytes

AutorMartínez de Mena, Raquel ; Hernández, Arturo; Obregón, María Jesús
Palabras claveNorepinephrine
Uncoupling protein-1
Fecha de publicaciónmay-2002
EditorAmerican Physiological Society
CitaciónAJP - Endocrinology and Metabolism 282(5): E1119-E1127 (2002)
ResumenType II 5'-iodothyronine deiodinase (D2), produces triiodothyronine (T(3)) and is stimulated by cold exposure via norepinephrine (NE) release in brown adipose tissue. Cultured rat brown adipocytes require T(3) for the adrenergic stimulation of D2 activity. D2 mRNA expression in cultured brown adipocytes is undetectable with the use of basal conditions or NE without T(3). Full D2 expression is achieved using NE + T(3), especially after prolonged T(3) exposure. beta(3)-Adrenergic agonists mimic the NE action, whereas cAMP analogs do not. Prolonged exposure to T(3) alone increases D2 mRNA. High T(3) doses (500 nM) inhibit the adrenergic stimulation of D2 activity while increasing D2 mRNA. The effects obtained with NE + T(3) or T(3) alone are suppressed by actinomycin, but not by cycloheximide, which leads to accumulation of short D2 mRNA transcripts. Prolonged or short exposure to T(3) did not change D2 mRNA half-life, but T(3) seemed to elongate it. In conclusion, T(3) is an absolute requirement for the adrenergic stimulation of D2 mRNA in brown adipocytes. T(3) upregulates D2 mRNA, an effect that might involve stimulation of factors required for transcription or for stabilization of D2 mRNA.
Descripción10 pages, 6 figures.
Versión del editorhttp://dx.doi.org/10.1152/ajpendo.00433.2001
URIhttp://hdl.handle.net/10261/24502
DOI10.1152/ajpendo.00433.2001
ISSN0193-1849
Aparece en las colecciones: (IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.