Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/244857
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Structural analysis of the regulation of the DYNLL/LC8 binding to Nek9 by phosphorylation |
Autor: | Gallego, Pablo; Velázquez-Campoy, Adrián; Regué, Laura; Roig, Joan CSIC ORCID ; Reverter, David | Fecha de publicación: | 26-abr-2013 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 288: 12283-12294-12294 (2013) | Resumen: | The NIMA family protein kinases Nek9/Nercc1, Nek6, and Nek7 constitute a signaling module activated in early mitosis involved in the control of spindle organization. DYNLL/LC8 (dynein light chain 8) was originally described as a component of the dynein complex, but the recent discovery of multiple interaction partners for LC8 has suggested that it has a general role as a dimerization hub that organizes different protein partners. Recent experiments suggested that LC8 binding to Nek9 was regulated by Nek9 autophosphorylation on Ser944, a residue immediately located N-terminal to the LC8 conserved (K/R)chi;-TQT binding motif, and that this was crucial for the control of signal transduction through the Nek/Nek6/7 module. In the present work, we present two crystal structures of LC8 with a peptide corresponding to the Nek9 binding region with and without a phosphorylation on Ser944. Structural analysis of LC8 with both Nek9 peptides, together with different biophysical experiments, explains the observed diminished binding affinity of Nek9 to LC8 upon phosphorylation on Ser944 within the Nek9 sequence, thus shedding light into a novel phosphorylation regulatory mechanism that interferes with LC8 protein-protein complex formation. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. | Descripción: | 12 pags, 6 figs, 3 tabs | Versión del editor: | http://dx.doi.org/10.1074/jbc.M113.459149 | URI: | http://hdl.handle.net/10261/244857 | DOI: | 10.1074/jbc.M113.459149 | Identificadores: | doi: 10.1074/jbc.M113.459149 issn: 0021-9258 |
Aparece en las colecciones: | (IQF) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Structural_Analysis.pdf | 2,26 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
41
checked on 17-abr-2024
Download(s)
56
checked on 17-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons