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Requirement of nuclear factor kB for the constitutive expression of nitric oxide synthase-2 and cyclooxygenase-2 in rat trophoblasts

AuthorsCallejas, Nuria A.; Casado, Marta CSIC ORCID ; Boscá, Lisardo CSIC ORCID CVN ; Martín-Sanz, Paloma CSIC ORCID
Nitric oxide
Cyclooxygenase 2
Nuclear factor kB
Issue DateSep-1999
PublisherCompany of Biologists
CitationJournal of Cell Science 112(18): 3147-3155 (1999)
AbstractRecently isolated trophoblasts express nitric oxide synthase 2 (NOS-2) and cyclooxygenase 2 (COX-2), decreasing the levels of the corresponding mRNAs when the cells were maintained in culture. The sustained expression of COX-2 and NOS-2 in trophoblasts was dependent on the activation of nuclear factor kappaB (NF-kappaB) since proteasome inhibitors and antioxidants that abrogated NF-kappaB activity suppressed the induction of both genes. The time-dependent fall of the mRNA levels of NOS-2 and COX-2 paralleled the inhibition of NF-kappaB, determined by electrophoretic mobility shift assays, and the increase of the IkappaBalpha and IkappaBbeta inhibitory proteins. Isolated trophoblasts synthesized reactive oxygen intermediates (ROI), a process impaired after culturing the cells, and that might be involved in the NF-kappaB activation process. Moreover, treatment of recently isolated cells with ROI scavengers suppressed the expression of COX-2 and NOS-2. Challenge of trophoblasts with interleukin-1beta up-regulated the expression of both proteins, an effect that was potentiated by lipopolysaccharide. These results indicate that the physiological expression of NOS-2 and COX-2 in trophoblasts involves a sustained activation of NF-kappaB which inhibition abrogates the inducibility of both genes.
Description9 pages, 6 figures, 3 tables.
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Appears in Collections:(IIBM) Artículos

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