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Título

Crystallization and preliminary X-ray diffraction analysis of the fructofuranosidase from Schwanniomyces occidentalis

AutorPolo, Aitana; Álvaro-Benito, Miguel; Fernández Lobato, María; Sanz-Aparicio, J.
Palabras claveSchwanniomyces occidentalis
X-ray crystallographic analyses
Fecha de publicación18-may-2010
EditorBlackwell Publishing
CitaciónActa Cryst. (2009). F65, 1162–1165
ResumenSchwanniomyces occidentalis invertase is an extracellular enzyme that releases -fructose from the nonreducing termini of various -d-fructofuranoside substrates. Its ability to produce 6-kestose by transglycosylation makes this enzyme an interesting research target for applications in industrial biotechnology. The enzyme has been expressed in Saccharomyces cerevisiae. Recombinant and wildtype forms, which showed different glycosylation patterns, were crystallized by vapour-diffusion methods. Although crystallization trials were conducted on both forms of the protein, crystals suitable for X-ray crystallographic analyses were only obtained from the wild-type enzyme. The crystals belonged to space group P212121, with unit-cell parameters a = 105.78, b = 119.49, c = 137.68 A ° . A diffraction data set was collected using a synchrotron source. Self-rotation function and sedimentation-velocity experiments suggested that the enzyme was dimeric with twofold symmetry.
Versión del editorhttp://dx.doi.org/10.1107/S1744309109039384
URIhttp://hdl.handle.net/10261/24450
DOI10.1107/S1744309109039384
ISSN1744-3091
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