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Título

The pancreatic homeodomain transcription factor IDX1/IPF1 is expressed in neural cells during brain development

AutorPérez-Villamil, Beatriz; Schwartz, Petra T.; Vallejo, Mario
Fecha de publicaciónago-1999
EditorEndocrine Society
CitaciónEndocrinology 140(8): 3857-3860 (1999)
ResumenExpression of the homeodomain transcription factor IDX1/IPF1 has been shown to be restricted to cells in the developing foregut that form the pancreatic primordium. In the adult, IDX1/IPF1 is expressed in the duodenum and pancreatic islets. The IDX1/IPF1 gene is required for pancreatic development, and in the human, heterozygous mutations have been linked to diabetes mellitus. In the present communication, we report that IDX1/IPF1 is expressed in discrete cells of the rat central nervous system during embryonic development. Using RT-PCR, IDX1/IPF1 mRNA was detected in neural precursor RC2.E10 cells, as well as in both forebrain and hindbrain of developing rats at embryonic day 15 (E15). The presence of IDX1/IPF1 protein was confirmed by Western immunoblotting. Immunohistochemical analyses of sagittal sections of E15 rats demonstrated the presence of scattered IDX1/IPF1-immunopositive cells in the forebrain. Finally, electrophoretic mobility shift assays using nuclear extracts from neural cells revealed the presence of IDX1/IPF1 bound to a putative homeodomain protein DNA-binding site present in the promoter of the glial fibrillary acidic protein gene. Our results suggest that IDX1/IPF1 may have previously unsuspected extrapancreatic functions during development of neural cells in the central nervous system.
Descripción4 pages, 4 figures.
Versión del editorhttp://dx.doi.org/10.1210/en.140.8.3857
URIhttp://hdl.handle.net/10261/24429
DOI10.1210/en.140.8.3857
ISSN0013-7227
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