English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24395
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Src family kinases are required for prolactin induction of cell proliferation

AutorFresno Vara, Juan Ángel ; Domínguez-Cáceres, Mª Aurora; Silva, Augusto ; Martín-Pérez, Jorge
Fecha de publicaciónjul-2001
EditorAmerican Society for Cell Biology
CitaciónMolecular Biology of the Cell 12(7): 2171-2183 (2001)
ResumenProlactin (PRL) is a pleiotropic cytokine promoting cellular proliferation and differentiation. Because PRL activates the Src family of tyrosine kinases (SFK), we have studied the role of these kinases in PRL cell proliferation signaling. PRL induced [(3)H]thymidine incorporation upon transient transfection of BaF-3 cells with the PRL receptor. This effect was inhibited by cotransfection with the dominant negative mutant of c-Src (K>A295/Y>F527, SrcDM). The role of SFK in PRL-induced proliferation was confirmed in the BaF-3 PRL receptor-stable transfectant, W53 cells, where PRL induced Fyn and Lyn activation. The SFK-selective inhibitors PP1/PP2 and herbimycin A blocked PRL-dependent cell proliferation by arresting the W53 cells in G1, with no evident apoptosis. In parallel, PP1/PP2 inhibited PRL induction of cell growth-related genes c-fos, c-jun, c-myc, and odc. These inhibitors have no effect on PRL-mediated activation of Ras/Mapk and Jak/Start pathways. In contrast, they inhibited the PRL-dependent stimulation of the SFKs substrate Sam68, the phosphorylation of the tyrosine phosphatase Shp2, and the PI3K-dependent Akt and p70S6k serine kinases. Consistently, transient expression of SrcDM in W53 cells also blocked PRL activation of Akt. These results demonstrate that activation of SFKs is required for cell proliferation induced by PRL.
Descripción13 pages, 9 figures.
Versión del editorhttp://www.molbiolcell.org/cgi/content/full/12/7/2171
URIhttp://hdl.handle.net/10261/24395
ISSN1059-1524
Aparece en las colecciones: (IIBM) Artículos
(CIB) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Src family.pdf499,99 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.