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Title

N-type inactivation of the potassium channel KcsA by the Shaker B "ball" Peptide: Mapping the inactivating peptide-binding epitope

AuthorsMolina, Maria L.; Barrera, Francisco N.; Encinar, José Antonio; Renart, M. Lourdes; Fernández, Asia M.; Poveda, José Antonio; Santoro, Jorge CSIC; Bruix, M. CSIC ORCID; Gavilanes, Francisco; Fernández-Ballester, Gregorio; Neira, José L.; González-Ros, José M.
Issue Date27-Jun-2008
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 283: 18076-18085 (2008)
AbstractThe effects of the inactivating peptide from the eukaryotic Shaker B K + channel (the ShB peptide) on the prokaryotic KcsA channel have been studied using patch clamp methods. The data show that the peptide induces rapid, N-type inactivation in KcsA through a process that includes functional uncoupling of channel gating. We have also employed saturation transfer difference (STD) NMR methods to map the molecular interactions between the inactivating peptide and its channel target. The results indicate that binding of the ShB peptide to KcsA involves the ortho and meta protons of Tyr 8, which exhibit the strongest STD effects; the C4H in the imidazole ring of His16; the methyl protons of Val4, Leu 7, and Leu10 and the side chain amine protons of one, if not both, the Lys18 and Lys19 residues. When a noninactivating ShB-L7E mutant is used in the studies, binding to KcsA is still observed but involves different amino acids. Thus, the strongest STD effects are now seen on the methyl protons of Val4 and Leu10, whereas His16 seems similarly affected as before. Conversely, STD effects on Tyr8 are strongly diminished, and those on Lys18 and/or Lys19 are abolished. Additionally, Fourier transform infrared spectroscopy of KcsA in presence of 13C-labeled peptide derivatives suggests that the ShB peptide, but not the ShB-L7E mutant, adopts a β-hairpin structure when bound to the KcsA channel. Indeed, docking such a β-hairpin structure into an open pore model for K+ channels to simulate the inactivating peptide/channel complex predicts interactions well in agreement with the experimental observations. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.
Description10 pags, 6 figs
Publisher version (URL)http://dx.doi.org/10.1074/jbc.M710132200
URIhttp://hdl.handle.net/10261/243745
Identifiersdoi: 10.1074/jbc.M710132200
issn: 0021-9258
Appears in Collections:(IQFR) Artículos

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