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dc.contributor.author | Díaz-Guerra, María José | - |
dc.contributor.author | Castrillo, Antonio | - |
dc.contributor.author | Martín-Sanz, Paloma | - |
dc.contributor.author | Boscá, Lisardo | - |
dc.date.accessioned | 2010-05-17T10:03:20Z | - |
dc.date.available | 2010-05-17T10:03:20Z | - |
dc.date.issued | 1999-05-15 | - |
dc.identifier.citation | Journal of Immunology 162(10): 6184-6190 (1999) | en_US |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | http://hdl.handle.net/10261/24357 | - |
dc.description | 8 pages, 7 figures. | en_US |
dc.description.abstract | Triggering of the macrophage cell line RAW 264.7 with LPS promotes a transient activation of phosphatidylinositol 3-kinase (PI3-kinase). Incubation of activated macrophages with wortmannin and LY294002, two inhibitors of PI3-kinase, increased the amount of inducible nitric oxide synthase (iNOS) and the synthesis of nitric oxide. Treatment with wortmannin promoted a prolonged activation of NF-{kappa}B in LPS-treated cells as well as an increase in the promoter activity of the iNOS gene as deduced from transfection experiments using a 1.7-kb fragment of the 5' flanking region of the iNOS gene. Cotransfection of cells with a catalytically active p110 subunit of PI3-kinase impaired the responsiveness of the iNOS promoter to LPS stimulation, whereas transfection with a kinase-deficient mutant of p110 maintained the up-regulation in response to wortmannin. These results indicate that PI3-kinase plays a negative role in the process of macrophage activation and suggest that this enzyme might participate in the mechanism of action of antiinflammatory cytokines. | en_US |
dc.description.sponsorship | This work was supported by Grants PM95-0007 and PM98-0120 from Dirección General de Investigación Científica y Técnica, Spain. | en_US |
dc.format.extent | 617545 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | American Association of Immunologists | en_US |
dc.rights | closedAccess | en_US |
dc.title | Negative regulation by phosphatidylinositol 3-Kinase of inducible nitric oxide synthase expression in macrophages | en_US |
dc.type | artículo | en_US |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://www.jimmunol.org/cgi/content/full/162/10/6184 | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
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