English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24323
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Protein kinase Cϵ is required for macrophage activation and defense against bacterial infection

AutorCastrillo, Antonio ; Parker, Peter J.; Boscá, Lisardo
Palabras claveNitric oxide
Protein kinase C
Nitric oxide synthase
Macrophage activation
Bacterial infection
Fecha de publicación5-nov-2001
EditorRockefeller University Press
CitaciónJournal of Experimental Medicine 194(9): 1231-1242 (2001)
ResumenTo assess directly the role of protein kinase C (PKC)epsilon in the immune system, we generated mice that carried a homozygous disruption of the PKCepsilon locus. PKCepsilon(-/-) animals appeared normal and were generally healthy, although female mice frequently developed a bacterial infection of the uterus. Macrophages from PKCepsilon(-/-) animals demonstrated a severely attenuated response to lipopolysaccharide (LPS) and interferon (IFN)gamma, characterized by a dramatic reduction in the generation of NO, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-1beta. Further analysis revealed that LPS-stimulated macrophages from PKCepsilon(-/-) mice were deficient in the induction of nitric oxide synthase (NOS)-2, demonstrating a decrease in the activation of IkappaB kinase, a reduction in IkappaB degradation, and a decrease in nuclear factor (NF)kappaB nuclear translocation. After intravenous administration of Gram-negative or Gram-positive bacteria, PKCepsilon(-/-) mice demonstrated a significantly decreased period of survival. This study provides direct evidence that PKCepsilon is critically involved at an early stage of LPS-mediated signaling in activated macrophages. Furthermore, we demonstrate that in the absence of PKCepsilon, host defense against bacterial infection is severely compromised, resulting in an increased incidence of mortality.
Descripción12 pages, 7 figures.-- et al.
Versión del editorhttp://dx.doi.org/10.1084/jem.194.9.1231
URIhttp://hdl.handle.net/10261/24323
DOI10.1084/jem.194.9.1231
ISSN0022-1007
Aparece en las colecciones: (IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Protein kinase.pdf266,44 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.