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dc.contributor.authorWatts, Johnathan K.-
dc.contributor.authorMartín-Pintado, N.-
dc.contributor.authorGomez-Pinto, Irene-
dc.contributor.authorSchwartzentruber, Jeremy-
dc.contributor.authorPortella, Guillem-
dc.contributor.authorOrozco, Modesto-
dc.contributor.authorGonzález, Carlos-
dc.contributor.authorDamha, Masad J.-
dc.date.accessioned2021-06-09T10:40:03Z-
dc.date.available2021-06-09T10:40:03Z-
dc.date.issued2010-01-13-
dc.identifierdoi: 10.1093/nar/gkp1225-
dc.identifierissn: 0305-1048-
dc.identifier.citationNucleic Acids Research 38: 2498-2511 (2010)-
dc.identifier.urihttp://hdl.handle.net/10261/243026-
dc.description14 pags., 7 figs., 3 tabs.-
dc.description.abstractHybrids of RNA with arabinonucleic acids 2′F-ANA and ANA have very similar structures but strikingly different thermal stabilities. We now present a thorough study combining NMR and other biophysical methods together with state-of-the-art theoretical calculations on a fully modified 10-mer hybrid duplex. Comparison between the solution structure of 2′F-ANA•RNA and ANA•RNA hybrids indicates that the increased binding affinity of 2′F-ANA is related to several subtle differences, most importantly a favorable pseudohydrogen bond (2′F-purine H8) which contrasts with unfavorable 2′-OH-nucleobase steric interactions in the case of ANA. While both 2′F-ANA and ANA strands maintained conformations in the southern/eastern sugar pucker range, the 2′F-ANA strand's structure was more compatible with the A-like structure of a hybrid duplex. No dramatic differences are found in terms of relative hydration for the two hybrids, but the ANA•RNA duplex showed lower uptake of counterions than its 2′F-ANA•RNA counterpart. Finally, while the two hybrid duplexes are of similar rigidities, 2′F-ANA single strands may be more suitably preorganized for duplex formation. Thus the dramatically increased stability of 2′F-ANA•RNA and ANA•RNA duplexes is caused by differencesin at least four areas, of which structure and pseudohydrogen bonding are the most important. © The Author(s) 2010. Published by Oxford University Press.-
dc.description.sponsorshipSpanish Ministerio de Ciencia e Innovacion (grants CTQ2007-68014-C02-02 to CG and BIO2009-10964 to MO); Fundacion Marcelino Botin (grant to MO); Canadian Institutes for Health Research (grant to M.J.D.); Natural Sciences and Engineering Research Council of Canada (postgraduate scholarship to J.K.W.). Funding for open access charge: Canadian Institutes for Health Research.-
dc.languageeng-
dc.publisherOxford University Press-
dc.relationMICINN/CTQ2007-68014-C02-02-
dc.relationMICINN/CTQ2007-68014-C02-02-
dc.relationMICINN/BIO2009-10964-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.titleDifferential stability of 2′F-ANA•RNA and ANA•RNA hybrid duplexes: Roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility-
dc.typeartículo-
dc.identifier.doi10.1093/nar/gkp1225-
dc.relation.publisherversionhttp://dx.doi.org/10.1093/nar/gkp1225-
dc.date.updated2021-06-09T10:40:03Z-
dc.rights.licensehttp://creativecommons.org/licenses/ by-nc/2.5-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderCanadian Institutes of Health Research-
dc.contributor.funderNatural Sciences and Engineering Research Council of Canada-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000024es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000038es_ES
dc.identifier.pmid20071751-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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