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http://hdl.handle.net/10261/243026
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dc.contributor.author | Watts, Johnathan K. | - |
dc.contributor.author | Martín-Pintado, N. | - |
dc.contributor.author | Gomez-Pinto, Irene | - |
dc.contributor.author | Schwartzentruber, Jeremy | - |
dc.contributor.author | Portella, Guillem | - |
dc.contributor.author | Orozco, Modesto | - |
dc.contributor.author | González, Carlos | - |
dc.contributor.author | Damha, Masad J. | - |
dc.date.accessioned | 2021-06-09T10:40:03Z | - |
dc.date.available | 2021-06-09T10:40:03Z | - |
dc.date.issued | 2010-01-13 | - |
dc.identifier | doi: 10.1093/nar/gkp1225 | - |
dc.identifier | issn: 0305-1048 | - |
dc.identifier.citation | Nucleic Acids Research 38: 2498-2511 (2010) | - |
dc.identifier.uri | http://hdl.handle.net/10261/243026 | - |
dc.description | 14 pags., 7 figs., 3 tabs. | - |
dc.description.abstract | Hybrids of RNA with arabinonucleic acids 2′F-ANA and ANA have very similar structures but strikingly different thermal stabilities. We now present a thorough study combining NMR and other biophysical methods together with state-of-the-art theoretical calculations on a fully modified 10-mer hybrid duplex. Comparison between the solution structure of 2′F-ANA•RNA and ANA•RNA hybrids indicates that the increased binding affinity of 2′F-ANA is related to several subtle differences, most importantly a favorable pseudohydrogen bond (2′F-purine H8) which contrasts with unfavorable 2′-OH-nucleobase steric interactions in the case of ANA. While both 2′F-ANA and ANA strands maintained conformations in the southern/eastern sugar pucker range, the 2′F-ANA strand's structure was more compatible with the A-like structure of a hybrid duplex. No dramatic differences are found in terms of relative hydration for the two hybrids, but the ANA•RNA duplex showed lower uptake of counterions than its 2′F-ANA•RNA counterpart. Finally, while the two hybrid duplexes are of similar rigidities, 2′F-ANA single strands may be more suitably preorganized for duplex formation. Thus the dramatically increased stability of 2′F-ANA•RNA and ANA•RNA duplexes is caused by differencesin at least four areas, of which structure and pseudohydrogen bonding are the most important. © The Author(s) 2010. Published by Oxford University Press. | - |
dc.description.sponsorship | Spanish Ministerio de Ciencia e Innovacion (grants CTQ2007-68014-C02-02 to CG and BIO2009-10964 to MO); Fundacion Marcelino Botin (grant to MO); Canadian Institutes for Health Research (grant to M.J.D.); Natural Sciences and Engineering Research Council of Canada (postgraduate scholarship to J.K.W.). Funding for open access charge: Canadian Institutes for Health Research. | - |
dc.language | eng | - |
dc.publisher | Oxford University Press | - |
dc.relation | MICINN/CTQ2007-68014-C02-02 | - |
dc.relation | MICINN/CTQ2007-68014-C02-02 | - |
dc.relation | MICINN/BIO2009-10964 | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | Differential stability of 2′F-ANA•RNA and ANA•RNA hybrid duplexes: Roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1093/nar/gkp1225 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1093/nar/gkp1225 | - |
dc.date.updated | 2021-06-09T10:40:03Z | - |
dc.rights.license | http://creativecommons.org/licenses/ by-nc/2.5 | - |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | - |
dc.contributor.funder | Canadian Institutes of Health Research | - |
dc.contributor.funder | Natural Sciences and Engineering Research Council of Canada | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000024 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004837 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000038 | es_ES |
dc.identifier.pmid | 20071751 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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Differential stability.pdf | 5,89 MB | Unknown | Visualizar/Abrir |
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