English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24245
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 11 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL

A Method for the Generation of Ectromelia Virus (ECTV) Recombinant: In Vivo Analysis of ECTV vCD30 Deletion Mutants

AutorAlejo, Alí; Saraiva, Margarida; Ruiz-Arguello, Maria Begoña; Viejo-Borbolla, Abel; Fernández de Marco, M. del Mar; Salguero, Francisco J.; Alcamí, Antonio
Palabras claveEctromelia Virus
ECTV vCD30 Deletion
Fecha de publicación13-abr-2009
EditorPublic Library of Science
CitaciónPLoS ONE 4(4): e5175.
ResumenBackground: Ectromelia virus (ECTV) is the causative agent of mousepox, a lethal disease of mice with similarities to human smallpox. Mousepox progression involves replication at the initial site of infection, usually the skin, followed by a rapid spread to the secondary replicative organs, spleen and liver, and finally a dissemination to the skin, where the typical rash associated with this and other orthopoxviral induced diseases appears. Case fatality rate is genetically determined and reaches up to 100% in susceptible mice strains. Like other poxviruses, ECTV encodes a number of proteins with immunomodulatory potential, whose role in mousepox progression remains largely undescribed. Amongst these is a secreted homologue of the cellular tumour necrosis factor receptor superfamily member CD30 which has been proposed to modulate a Th1 immune response in vivo. Methodology/Principal Findings: To evaluate the contribution of viral CD30 (vCD30) to virus pathogenesis in the infected host, we have adapted a novel transient dominant method for the selection of recombinant ECTVs. Using this method, we have generated an ECTV vCD30 deletion mutant, its corresponding revertant control virus as well as a virus encoding the extracellular domain of murine CD30. These viruses contain no exogenous marker DNA sequences in their genomes, as opposed to other ECTVs reported up to date. Conclusions/Significance: We show that the vCD30 is expressed as a secreted disulfide linked trimer and that the absence of vCD30 does not impair mousepox induced fatality in vivo. Replacement of vCD30 by a secreted version of mouse CD30 caused limited attenuation of ECTV. The recombinant viruses generated may be of use in the study of the role of the cellular CD30-CD30L interaction in the development of the immune response. The method developed might be useful for the construction of ECTV mutants for the study of additional genes.
Versión del editorhttp://dx.doi.org/10.1371/journal.pone.0005175
Aparece en las colecciones: (CBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
AAli_PlosOne_5175.pdf1,1 MBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.