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Title

Kv1.3 channels are novel determinants of macrophage-dependent endothelial dysfunction in angiotensin II-induced hypertension in mice

AuthorsOlivencia, Miguel A.; Martínez-Casales, Marta; Peraza, Diego A.; García-Redondo, Ana B.; Mondéjar‐Parreño, Gema; Hernanz, Raquel; Salaices, Mercedes; Cogolludo, Angel; Pennington, Michael W.; Valenzuela, Carmen CSIC ORCID; Briones, Ana M.
KeywordsAngiotensin II
Endothelial dysfunction
KV1.3 channels
Macrophages
Vascular myocytes
Issue DateApr-2021
PublisherBritish Pharmacological Society
John Wiley & Sons
CitationBritish Journal of Pharmacology 178(8): 1836-1854 (2021)
Abstract[Background and Purpose]: KV1.3 channels are expressed in vascular smooth muscle cells (VSMCs), where they contribute to proliferation rather than contraction and participate in vascular remodelling. KV1.3 channels are also expressed in macrophages, where they assemble with KV1.5 channels (KV1.3/KV1.5), whose activation generates a KV current. In macrophages, the KV1.3/KV1.5 ratio is increased by classical activation (M1). Whether these channels are involved in angiotensin II (AngII)-induced vascular remodelling, and whether they can modulate the macrophage phenotype in hypertension, remains unknown. We characterized the role of KV1.3 channels in vascular damage in hypertension.
[Experimental Approach]: We used AngII-infused mice treated with two selective KV1.3 channel inhibitors (HsTX[R14A] and [EWSS]ShK). Vascular function and structure were measured using wire and pressure myography, respectively. VSMC and macrophage electrophysiology were studied using the patch-clamp technique; gene expression was analysed using RT-PCR.
[Key Results]: AngII increased KV1.3 channel expression in mice aorta and peritoneal macrophages which was abolished by HsTX[R14A] treatment. KV1.3 inhibition did not prevent hypertension, vascular remodelling, or stiffness but corrected AngII-induced macrophage infiltration and endothelial dysfunction in the small mesenteric arteries and/or aorta, via a mechanism independent of electrophysiological changes in VSMCs. AngII modified the electrophysiological properties of peritoneal macrophages, indicating an M1-like activated state, with enhanced expression of proinflammatory cytokines that induced endothelial dysfunction. These effects were prevented by KV1.3 blockade.
[Conclusions and Implications]: We unravelled a new role for KV1.3 channels in the macrophage-dependent endothelial dysfunction induced by AngII in mice which might be due to modulation of macrophage phenotype.
Publisher version (URL)http://dx.doi.org/10.1111/bph.15407
URIhttp://hdl.handle.net/10261/242086
DOIhttp://dx.doi.org/10.1111/bph.15407
ISSN0007-1188
E-ISSN1476-5381
Appears in Collections:(IIBM) Artículos
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