English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/24198
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Apoptosis induced by Rac GTPase correlates with induction of FasL and ceramides production

AuthorsEmbade, Nieves; Valerón, Pilar F.; Aznar, Salvador; López-Collazo, Eduardo; Lacal, Juan Carlos
Issue DateDec-2000
PublisherAmerican Society for Cell Biology
CitationMolecular Biology of the Cell 11(12): 4347-4358 (2000)
AbstractRho proteins, members of the Ras superfamily of GTPases, are critical elements in signal transduction pathways governing cell proliferation and cell death. Different members of the family of human Rho GTPases, including RhoA, RhoC, and Rac1, participate in the regulation of apoptosis in response to cytokines and serum deprivation in different cell systems. Here, we have characterized the mechanism of apoptosis induced by Rac1 in NIH 3T3 cells. It requires protein synthesis and caspase-3 activity, but it is independent of the release of cytochrome c from mitochondria. Moreover, an increase in mitochondria membrane potential and the production of reactive oxygen species was observed. Rac1-induced apoptosis was related to the simultaneous increase in ceramide production and synthesis of FasL. Generation of FasL may be mediated by transcriptional regulation involving both c-Jun amino terminal kinase as well as nuclear factor-kappa B-dependent signals. None of these signals, ceramides or FasL, was sufficient to induce apoptosis in the parental cell line, NIH 3T3 cells. However, any of them was sufficient to induce apoptosis in the Rac1-expressing cells. Finally, inhibition of FasL signaling drastically reduced apoptosis by Rac1. Thus, Rac1 seems to induce apoptosis by a complex mechanism involving the generation of ceramides and the de novo synthesis of FasL. These results suggest that apoptosis mediated by Rac1 results from a signaling mechanism that involves biochemical and transcriptional events under control of Rac1.
Description12 pages, 9 figures.
Publisher version (URL)http://dx.doi.org/10.1091/mbc.11.12.4347
URIhttp://hdl.handle.net/10261/24198
DOI10.1091/mbc.11.12.4347
ISSN1059-1524
Appears in Collections:(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
Apoptosis induced.pdf457,74 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.