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Enantiomer analysis of chiral lactones in foods by on-line coupled reversed-phase liquid chromatography—gas chromatography

AuthorsBlanch, Gracia P. CSIC ORCID ; Ruiz del Castillo, M. Luisa CSIC ORCID ; Herraiz Carasa, Marta CSIC ORCID
Issue Date1998
PublisherOxford University Press
CitationJournal of Chromatographic Science 36(12): 589–594 (1998)
AbstractA new application is proposed for the on-line coupling of reversed-phase liquid chromatography to gas chromatography (RPLC—GC) that allows the GC chirospecific analysis of γ-lactones in fruits and commercially available fruit-containing products. The use of a programmed temperature vaporizer as an interface with the system makes the transfer of large volume fractions (i.e., 2520 µL) of aqueous eluents from LC to GC possible (speed of sample transfer, 1800 µL/min). Relative standard deviations obtained for the investigated lactones under the experimental conditions vary from 7 to 14%. The described system enlarges the LC—GC application field and overcomes the limitations reported thus far concerning the use of typical normal-phase eluents (i.e., the transfer of rather small volume fractions at low speeds of sample introduction).
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