Saccharomyces cerevisiae GPI10, the functional homologue of human PIG-B, is required for glycosylphosphatidylinositol-anchor synthesis

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Campo DC	Valor	Lengua/Idioma
dc.contributor.author	Escribano, M. Victoria	-
dc.contributor.author	Mazón, María J.	-
dc.date.accessioned	2010-05-11T09:28:40Z	-
dc.date.available	2010-05-11T09:28:40Z	-
dc.date.issued	1998-05-15	-
dc.identifier.citation	Biochemical Journal 332(1): 153-159 (1998)	en_US
dc.identifier.issn	0264-6021	-
dc.identifier.uri	http://hdl.handle.net/10261/24168	-
dc.description	7 pages, 5 figures, 2 tables.-- et al.	en_US
dc.description.abstract	An increasing number of plasma membrane proteins have been shown to be attached to the membrane via a glycosylphosphatidylinositol (GPI) moiety. All eukaryotes share a highly conserved GPI-core structure EthN-P-Man3-GlcN-PI, where EthN is ethanolamine. We have identified a protein encoded by the yeast open reading frame YGL142C that shares 33% identity with the human Pig-B protein. Deletion of this essential gene leads to a block in GPI anchor biosynthesis. We therefore named the gene GPI10. Gpi10p and Pig-B are functional homologues and the lethal deletion of GPI10 can be rescued by expression of the PIG-B cDNA. As found for PIG-B mutant cells, gpi10 deletant cells cannot attach the third mannose in an alpha-1,2 linkage to the GPI core-structure intermediate. Overexpression of GPI10 gives partial resistance to the GPI-synthesis inhibitor YW3548, suggesting that this gene product may affect the target of the inhibitor.	en_US
dc.description.sponsorship	This work was supported by grants from the Swiss National Science Foundation, the Canton of Basel-Stadt and the Swiss Federal Office for Education and Science to H. R., and by a grant of HFSPO to H. R., R. T. S. and T. K. M.J.M. was supported by grants UE (BIO4-CT95-0080) and CICYT (BIO96-2461-CE). M.V. E. thanks FEBS for a short term fellowship. R. T. S. was supported by the Deutsche Forschungsgemeinschaft, Hessische Ministerium für Kultur und Wissenschaft, Fonds der Chemischen Industrie and Stiftung P. E. Kempkes.	en_US
dc.format.extent	491385 bytes	-
dc.format.mimetype	application/pdf	-
dc.language.iso	eng	en_US
dc.publisher	Biochemical Society	en_US
dc.rights	closedAccess	en_US
dc.title	Saccharomyces cerevisiae GPI10, the functional homologue of human PIG-B, is required for glycosylphosphatidylinositol-anchor synthesis	en_US
dc.type	artículo	en_US
dc.description.peerreviewed	Peer reviewed	en_US
dc.relation.publisherversion	http://www.biochemj.org/bj/332/0153/bj3320153.htm	en_US
dc.type.coar	http://purl.org/coar/resource_type/c_6501	es_ES
item.openairecristype	http://purl.org/coar/resource_type/c_18cf	-
item.fulltext	No Fulltext	-
item.cerifentitytype	Publications	-
item.openairetype	artículo	-
item.languageiso639-1	en	-
item.grantfulltext	none	-
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