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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Shima, David T. | - |
dc.contributor.author | Cabrera, Noemí | - |
dc.contributor.author | Pepperkok, Rainer | - |
dc.contributor.author | Warren, Graham | - |
dc.date.accessioned | 2010-05-11T08:53:31Z | - |
dc.date.available | 2010-05-11T08:53:31Z | - |
dc.date.issued | 1998-05-18 | - |
dc.identifier.citation | Journal of Cell Biology 141(4): 955-966 (1998) | en_US |
dc.identifier.issn | 0021-9525 | - |
dc.identifier.uri | http://hdl.handle.net/10261/24162 | - |
dc.description | 12 pages, 8 figures. | en_US |
dc.description.abstract | During mitosis, the ribbon of the Golgi apparatus is transformed into dispersed tubulo-vesicular membranes, proposed to facilitate stochastic inheritance of this low copy number organelle at cytokinesis. Here, we have analyzed the mitotic disassembly of the Golgi apparatus in living cells and provide evidence that inheritance is accomplished through an ordered partitioning mechanism. Using a Sar1p dominant inhibitor of cargo exit from the endoplasmic reticulum (ER), we found that the disassembly of the Golgi observed during mitosis or microtubule disruption did not appear to involve retrograde transport of Golgi residents to the ER and subsequent reorganization of Golgi membrane fragments at ER exit sites, as has been suggested. Instead, direct visualization of a green fluorescent protein (GFP)-tagged Golgi resident through mitosis showed that the Golgi ribbon slowly reorganized into 1-3-micron fragments during G2/early prophase. A second stage of fragmentation occurred coincident with nuclear envelope breakdown and was accompanied by the bulk of mitotic Golgi redistribution. By metaphase, mitotic Golgi dynamics appeared to cease. Surprisingly, the disassembly of mitotic Golgi fragments was not a random event, but involved the reorganization of mitotic Golgi by microtubules, suggesting that analogous to chromosomes, the Golgi apparatus uses the mitotic spindle to ensure more accurate partitioning during cytokinesis. | en_US |
dc.description.sponsorship | D.T. Shima is a Hitchings-Elion fellow supported by the Burroughs Wellcome Fund and N. Cabrera-Poch is a recipient of a Postdoctoral Fellowship from the Spanish Ministry of Education and Culture. | en_US |
dc.format.extent | 3835938 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Rockefeller University Press | en_US |
dc.rights | openAccess | en_US |
dc.title | An ordered inheritance strategy for the Golgi apparatus: visualization of mitotic disassembly reveals a role for the mitotic spindle | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1083/jcb.141.4.955 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://dx.doi.org/10.1083/jcb.141.4.955 | en_US |
dc.identifier.pmid | 9585414 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
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955.full.pdf | 3,75 MB | Adobe PDF | Visualizar/Abrir |
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