English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24149
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Nitric oxide induces tyrosine nitration and release of cytochrome c preceding an increase of mitochondrial transmembrane potential in macrophages

AutorHortelano, Sonsoles; Álvarez, Alberto; Boscá, Lisardo
Palabras claveApoptosis
Nitric oxide synthase
Mitochondria
Fecha de publicacióndic-1999
EditorFederation of American Societies for Experimental Biology
CitaciónFaseb Journal 13(15): 2311-2317 (1999)
ResumenTreatment of elicited peritoneal macrophages or the macrophage cell line RAW 264.7 with high concentrations of nitric oxide donors is followed by apoptotic cell death. Analysis of the changes in the mitochondrial transmembrane potential ({Delta}{Psi}m) with specific fluorescent probes showed a rapid and persistent increase of {Delta}{Psi}m, a potential that usually decreases in cells undergoing apoptosis through mitochondrial-dependent mechanisms. Using confocal microscopy, the release of cytochrome c from the mitochondria to the cytosol was characterized as an early event preceding the rise of {Delta}{Psi}m. The cytochrome c from cells treated with nitric oxide donors was modified chemically, probably through the formation of nitrotyrosine residues, suggesting the synthesis of peroxynitrite in the mitochondria. These results indicate that nitric oxide-dependent apoptosis in macrophages occurs in the presence of a sustained increase of {Delta}{Psi}m, and that the chemical modification and release of cytochrome c from the mitochondria precede the changes of {Delta}{Psi}m.—Hortelano, S., Alvarez, A. M., Boscá, L. Nitric oxide induces tyrosine nitration and release of cytochrome c preceding an increase of mitochondrial transmembrane potential in macrophages.
Descripción7 pages, 5 figures.
Versión del editorhttp://www.fasebj.org/cgi/content/full/13/15/2311
URIhttp://hdl.handle.net/10261/24149
ISSN0892-6638
Aparece en las colecciones: (IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.