English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24136
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells

AutorLatasa, María Jesús ; Belandia, Borja ; Pascual, Ángel
Fecha de publicaciónjun-1998
EditorEndocrine Society
CitaciónEndocrinology 139(6): 2692-2698 (1998)
ResumenThe β-amyloid protein (Aβ), the major component of the senile plaques found in Alzheimer brains, derives from a larger β-amyloid precursor protein (APP). Alternative splicing of the APP gene yields three major APP messenger RNAs (mRNAs), which, in turn, give rise to the APP770, APP751, and APP695 protein isoforms. In this study we examined the effects of thyroid hormone on APP expression in N2a-β neuroblastoma cells. T3 caused a significant increase in the APP770 mRNA band, in detriment of the APP695 mRNA, which was proportionately reduced. In agreement with these results, T3 markedly altered the relative ratio of intracellular APP isoforms, increasing the amount of APP770 and causing an equivalent reduction of the immature APP695 isoform. In accordance with these results, the soluble APP695-derived form was specifically reduced in the culture medium obtained from T3-treated cells. In contrast, the increase in intracellular APP770 was not followed by an enhanced release of soluble derivatives of this isoform. These results suggest that T3 regulates not only APP gene splicing, but also the processing and secretion of the APP peptides. According to our results, thyroid hormone might play a role in the development of Alzheimer’s disease by modulating the intracellular and extracellular contents of APP isoforms.
Descripción7 pages, 6 figures.
Versión del editorhttp://dx.doi.org/10.1210/en.139.6.2692
URIhttp://hdl.handle.net/10261/24136
DOI10.1210/en.139.6.2692
ISSN0013-7227
Aparece en las colecciones: (IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Thyroid hormones.pdf330,51 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.