English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24128
Título

Splenic B lymphocyte programmed cell death is prevented by nitric oxide release through mechanisms involving sustained Bcl-2 levels

AutorGenaro, Ana M.; Hortelano, Sonsoles; Álvarez, Alberto; Martínez-Alonso, Carlos; Boscá, Lisardo
Palabras claveNitric oxide
B cells
Apoptosis
Bcl-2
Mhc-1
Fecha de publicaciónabr-1995
EditorAmerican Society for Clinical Investigation
CitaciónJournal of Clinical Investigation 95(4): 1884-1890 (1995)
ResumenIncubation of ex vivo cultured mature B cells in the presence of nitric oxide or nitric oxide-donor substances delays programmed cell death as determined by the appearance of DNA laddering in agarose gel electrophoresis or by flow-cytometry analysis of DNA. Nitric oxide also rescues B cells from antigen-induced apoptosis but fails to provide a co-stimulatory signal that converts the signal elicited by the antigen into a proliferative response. The protective effects of nitric oxide against programmed cell death can be reproduced by treatment of the cells with permeant analogues of cyclic GMP. Regarding the mechanisms by which nitric oxide prevents apoptosis in B cells, we have observed that nitric oxide release prevents the drop in the expression of the protooncogene bcl-2, both at the mRNA and protein levels, suggesting the existence of an unknown pathway that links nitric oxide signaling with Bcl-2 expression.
Descripción7 pages, 6 figures, 2 tables.
Versión del editorhttp://dx.doi.org/10.1172/JCI117869
URIhttp://hdl.handle.net/10261/24128
DOI10.1172/JCI117869
ISSN0021-9738
Aparece en las colecciones: (IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Splenic B.pdf1,58 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.