English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/241164
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

A thermostable DNA primase-polymerase from a mobile genetic element involved in defence against environmental DNA

AuthorsGarcía-Quintans, Nieves CSIC; Baquedano, Ignacio; Blesa, Alba CSIC ORCID CVN; Verdú, Carlos; Berenguer, José CSIC ORCID; Mencía, Mario CSIC ORCID
Issue Date18-Aug-2020
PublisherBlackwell Publishing
CitationEnvironmental Microbiology 22: 4647- 4657 (2020)
AbstractPrimase-polymerases (Ppol) are one of the few enzymes able to start DNA synthesis on ssDNA templates. The role of Thermus thermophilus HB27 Ppol, encoded along a putative helicase (Hel) within a mobile genetic element (ICETh2), has been studied. A mutant lacking Ppol showed no effects on the replication of the element. Also, no apparent differences in the sensitivity to DNA damaging agents and other stressors or morphological changes in the mutant cells were detected. However, the mutants lacking Ppol showed an increase in two to three orders of magnitude in their transformation efficiency with plasmids and genomic DNA acquired from the environment (eDNA), independently of its origin and G + C content. In contrast, no significant differences with the wild type were detected when the cells received the DNA from other T. thermophilus partners in conjugation-like mating experiments. The similarities of this behaviour with that shown by mutants lacking the Argonaute (ThAgo) protein suggests a putative partnership Ppol-ThAgo in the DNA–DNA interference mechanism of defence, although other eDNA defence mechanisms independent of ThAgo cannot be discarded.
Publisher version (URL)http://dx.doi.org/10.1111/1462-2920.15207
URIhttp://hdl.handle.net/10261/241164
Identifiersdoi: 10.1111/1462-2920.15207
issn: 1462-2920
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
BerenguerJ_AThermostableDNAPrimase.pdf708,1 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.