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Title

Targeted Investigation of the Neandertal Genome by Array-Based Sequence Capture.

AuthorsBurbano, Hernán A.; Hodges, Emily; Green, Richard E.; Briggs, Adrian W.; Krause, Johannes; Meyer, Matthias; Good, Jeffrey M.; Maricic, Tomislav; Johnson, Philip L. F.; Xuan, Zhenyu; Rooks, Michelle; Bhattacharjee, Arindam; Brizuela, Leonardo; Albert, Frank W.; Rasilla, Marco de la; Fortea, Javier; Rosas, Antonio ; Lachmann, Michael; Hannon, Gregory J.; Pääbo, Svante
Issue Date7-May-2010
PublisherAAS Division of Planetary Sciences
CitationSciencie 328(5979): 723-725 (2010)
AbstractIt is now possible to perform whole-genome shotgun sequencing as well as capture of specific genomic regions for extinct organisms. However, targeted resequencing of large parts of nuclear genomes has yet to be demonstrated for ancient DNA. Here we show that hybridization capture on microarrays can successfully recover more than a megabase of target regions from Neandertal DNA even in the presence of ~99.8% microbial DNA. Using this approach, we have sequenced ~14,000 protein-coding positions inferred to have changed on the human lineage since the last common ancestor shared with chimpanzees. By generating the sequence of one Neandertal and 50 present-day humans at these positions, we have identified 88 amino acid substitutions that have become fixed in humans since our divergence from the Neandertals.
Description3 pages, 2 figures.
Publisher version (URL)http://dx.doi.org/10.1126/science.1188046
URIhttp://hdl.handle.net/10261/24040
DOI10.1126/science.1188046
ISSN0036-8075
E-ISSN1095-9203
Appears in Collections:(MNCN) Artículos
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