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Stimulation of DNA synthesis by natural ceramide 1-phosphate

AuthorsGómez-Muñoz, Antonio; Frago, Laura M.; Álvarez, Luis; Varela-Nieto, Isabel
Issue Date15-Jul-1997
PublisherBiochemical Society
CitationBiochemical Journal 325(2): 435-440 (1997)
AbstractWe found that natural (long-chain) ceramide 1-phosphate can be dispersed into aqueous solution when dissolved in an appropriate mixture of methanol/dodecane (49:1, v/v). This solvent mixture facilitates the interaction of this phosphosphingolipid with cells. Under these conditions, incubation of EGFR T17 fibroblasts with natural ceramide 1-phosphate caused a potent stimulation of DNA synthesis. This effect was accompanied by an increase in the levels of proliferating-cell nuclear antigen. Concentrations of natural ceramide 1-phosphate that stimulated the synthesis of DNA did not inhibit adenylate cyclase activity, nor did they stimulate phospholipase D. Natural ceramide 1-phosphate did not alter the cellular phosphorylation state of tyrosine residues or of mitogen-activated protein kinase. Furthermore, natural ceramide 1-phosphate failed to induce the expression of the proto-oncogenes c-myc and c-fos. Both the stimulation of DNA synthesis and the induction of proliferating-cell nuclear antigen by natural ceramide 1-phosphate were inhibited by natural ceramides. This work suggests that the use of methanol and dodecane to deliver natural ceramide 1-phosphate to cells may be useful for elucidation of the biological function(s) and mechanism(s) of action of ceramide 1-phosphate.
Description6 pages, 6 figures, 1 table.
Publisher version (URL)http://www.biochemj.org/bj/325/0435/bj3250435.htm
Appears in Collections:(IIBM) Artículos
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