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C-erbA and v-erbA modulate growth and gene expression of a mouse glial precursor cell line

AutorIglesias, Teresa ; Llanos, Susana; López-Barahona, Mónica; Rodríguez-Peña, Ángeles ; Bernal, Juan ; Seliger, Barbara; Muñoz Terol, Alberto
Fecha de publicaciónjul-1994
EditorAmerican Association for Cancer Research
CitaciónCell Growth and Differentiation 5(7): 697-704 (1994)
ResumenThe c-erbAa protooncogene coding for the thyroid hormone (T3) receptor (TRa1 ) and the viral, mutated v-erbA oncogene were expressed in an immortal mouse glial cell line (B3.1 ) using retroviral vedors. c-erbAa expression led to a decrease in cell proliferation in high and low serum conditions, both in the presence and in the absence of T3. In serum-free medium, c-erbAexpressing cells (B3.1 + TRa1 ) were completely arrested, whereas cells expressing v-erbA (B3.1 + v-erbA) showed a higher DNA synthesis rate than normal B3.1 cells. Although proliferation of all three cell types was stimulated by platelet-derived growth factor and basic fibroblast growth factor, differences were also observed in the response to these agents. B3.1 + TRa1 cells were more sensitive to platelet-derived growth factor than B3.1 and B3.1 + v-erbA cells. In contrast, B3.1 cells responded to basic fibroblast growth factor better than B3.1 + TRa1 or B3.1 + v-erbA cells. Insulinlike growth factor I potentiated the action of plateletderived growth factor and basic fibroblast growth factor. Again, different responses to treatment with insulin-like growth factor I alone were observed; B3.1 + TRa1 cells did not respond to it, whereas B3.1 + v-erbA cells showed a dramatic stimulation by this agent. Interestingly, in the presence of 13, the blockade in B3.1 + TRa1 cell proliferation was accompanied by the down-regulation of the typical astrocytic genes, glial fibrillary acidic protein and vimentin. These hormone effects were not found in v-erbA-expressing cells. In addition, v-erbA inhibited the basal expression of the cyclic nucleotide phosphodiesterase gene, an oligodendrocytic marker. In summary, our results show that c-erbAa inhibits cell proliferation, whereas v-erbA has the opposite effect, causing an increased cell growth. Both genes also affect distinctly gene expression in B3.1 cells. Together, these data suggest a role for erbA genes in glial cell proliferation and differentiation.
Descripción8 pages, 7 figures.-- et al.
Versión del editorhttp://cgd.aacrjournals.org/cgi/content/abstract/5/7/697
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