Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/23926
Share/Export:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

The mutation DGT1-1 decreases glucose transport and alleviates carbon catabolite repression in Saccharomyces cerevisiae

AuthorsGamo, Francisco-Javier; Lafuente, María J.; Gancedo, Carlos CSIC ORCID
Issue DateDec-1994
PublisherAmerican Society for Microbiology
CitationJournal of Bacteriology 176(24): 7423-7429 (1994)
AbstractGlucose in ethanol-glycerol mixtures inhibits growth of Saccharomyces cerevisiae mutants lacking phosphoglycerate mutase. A suppressor mutation that relieved glucose inhibition was isolated. This mutation, DGT1-1 (decreasing glucose transport), was dominant and produced pleiotropic effects even in an otherwise wild-type background. Growth of the DGT1-1 mutant in glucose was dependent on respiration, and no ethanol was detected in the medium within 7 h of glucose addition. When grown on glucose, the mutant had a reduced glucose uptake and both the low- and high-affinity transport systems were affected. In galactose-grown cells, only the high-affinity glucose transport system was detected. This system had similar kinetic characteristics in the wild type and in the mutant. Catabolite repression of several enzymes was absent in the mutant during growth in glucose but not during growth in galactose. In contrast with the wild type, the mutant grown in glucose had high transcription of the glucose transporter gene SNF3 and no transcription of HXT1 and HXT3. Expression of multicopy plasmids carrying the HXT1, HXT2, or HXT3 gene allowed partial recovery of both fermentative capacity and catabolite repression in the mutant. The results suggest that DGT1 codes for a regulator of the expression of glucose transport genes. They also suggest that glucose flux might determine the levels of molecules implicated as signals in catbolite repression.
Description7 pages, 5 figures, 4 tables.
Publisher version (URL)http://jb.asm.org/cgi/reprint/176/24/7423
URIhttp://hdl.handle.net/10261/23926
ISSN0021-9193
Appears in Collections:(IIBM) Artículos

Files in This Item:
File Description SizeFormat
jbacter00042-0025.pdf1,42 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

Page view(s)

251
checked on May 16, 2022

Download(s)

321
checked on May 16, 2022

Google ScholarTM

Check


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.