English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/23890
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Insulin-like growth factor-I stimulates neurogenesis in chick retina by regulating expression of the alpha 6 integrin subunit

AutorFrade López, José María ; Martí, Elisa ; Bovolenta, Paola; Rodríguez-Peña, Ángeles ; Pérez-García, D.; Rohrer, Hermann; Edgar, David; Rodríguez-Tebar, Alfredo
Palabras claveRetina development
Neuron differentiation
Insulin-like growth factor I
Integrin subunit alpha 6
Chick
Fecha de publicaciónago-1996
EditorCompany of Biologists
CitaciónDevelopment 122(8): 2497-2506 (1996)
ResumenInsulin-like growth factor I (IGF-I) strongly stimulates the generation of differentiated neurons in cultures of neuroepithelial cells of the embryonic chick neural retina in the presence of a laminin-1 tissue culture substrate. Treatment of cultured neuroepithelial cells with IGF-I rapidly up-regulated the mRNA coding for the alpha 6 integrin subunit whereas specific reduction of alpha 6 subunit levels by treatment with an alpha 6 integrin antisense oligonucleotide resulted in reduced neuronal differentiation in vitro. Although IGF-I immunoreactivity is seen throughout the neural retina, expression of IGF-I mRNA is confined to the pigment epithelium during the period of neurogenesis in vivo. Neutralization of the endogenous IGF-I with a blocking antibody down-regulated levels of alpha 6 integrin mRNA and reduced the production of differentiated retinal neurons in vivo. These data indicate a role for IGF-I in the generation of retinal neurons mediated by the interaction of laminin with its alpha 6 integrin subunit-containing receptor.
Descripción10 pages, 9 figures, 1 table.
Versión del editorhttp://dev.biologists.org/content/122/8/2497.long
URIhttp://hdl.handle.net/10261/23890
ISSN0950-1991
Aparece en las colecciones: (IC) Artículos
(IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.