English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/23845
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 18 veces en Web of Knowledge®  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Regulation of malic-enzymes-gene expression by cAMP and retinoic acid in differentiating brown adipocytes

AutorHernández, Arturo; García-Jiménez, Custodia; Santisteban, Pilar ; Obregón, María Jesús
Fecha de publicaciónjul-1993
EditorWiley-Blackwell
CitaciónEuropean Journal of Biochemistry 215(2): 285-290 (1993)
ResumenBrown adipose tissue (BAT) is composed of highly specialized cells, whose main function is to produce heat under adrenergic stimulation, uncoupling oxidative phosphorylation. For this function, lipogenesis must be accurately regulated. Malic enzyme has a central role in lipogenesis and is strongly expressed in brown adipocytes. In this work, we study the modulation by adrenergic stimuli, cAMP effectors and retinoic acid on the induction produced by insulin and 3,5,3'-triiodothyronine on malic-enzyme-gene expression. Primary cultures of differentiating brown adipocytes have been used. The results obtained demonstrate that physiological doses of norepinephrine do not modify malic-enzyme mRNA levels when acting alone, but considerably reduce the induction produced by insulin, 3,5,3'-triiodothyronine or both together. Other cAMP inducers such as glucagon, forskolin or 8-bromo-cAMP, greatly inhibit both, basal and 3,5,3'-triiodothyronine-induced malic-enzyme-gene expression. Retinoic acid abolishes basal and also inhibits 3,5,3'-triiodothyronine-induced malic-enzyme-gene expression.
Descripción6 pages, 5 figures.-- Now known as FEBS Journal: Open Access content older than 1 year.
Versión del editorhttp://dx.doi.org/10.1111/j.1432-1033.1993.tb18033.x
URIhttp://hdl.handle.net/10261/23845
DOI10.1111/j.1432-1033.1993.tb18033.x
ISSN0014-2956
Aparece en las colecciones: (IIBM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
malic-enzymes-gene.pdf2,47 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.