Increased sensitivity to oxidative injury in chinese hamster ovary cells stably transfected with rat liver S-adenosylmethionine synthetase cDNA

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Campo DC	Valor	Lengua/Idioma
dc.contributor.author	Sánchez-Góngora, Estrella	-
dc.contributor.author	Álvarez, Luis	-
dc.contributor.author	Pajares, María A.	-
dc.contributor.author	García, Concepción	-
dc.contributor.author	Mato, José M.	-
dc.date.accessioned	2010-05-03T09:11:12Z	-
dc.date.available	2010-05-03T09:11:12Z	-
dc.date.issued	1996-11-01	-
dc.identifier.citation	Biochemical Journal 319(3): 767-773 (1996)	en_US
dc.identifier.issn	0264-6021	-
dc.identifier.uri	http://hdl.handle.net/10261/23819	-
dc.description	7 pages, 6 figures, 4 tables.-- et al.	en_US
dc.description.abstract	Chinese hamster ovary cells were stably transfected with rat liver S-adenosylmethionine synthetase cDNA. As a result, S-adenosylmethionine synthetase activity increased 2.3-fold, an effect that was accompanied by increased S-adenosylmethionine, a depletion of ATP and NAD levels, elevation of the S-adenosylmethionine/S-adenosylhomocysteine ratio (the methylation ratio), increased DNA methylation and polyamine levels (spermidine and spermine), and normal GSH levels. By contrast, the transfected cells showed normal growth curves and morphology. Exposure to an oxidative stress by the addition of H2O2 resulted in a greater consumption of ATP and NAD in the transfected cells than in the wild-type cells. In turn, cell killing by H2O2 was greater in the transfected cells than in the wild-type cells. This killing of Chinese hamster ovary cells by H2O2 involved the activation of poly(ADP-ribose) polymerase with the resultant loss of NAD and ATP. 3-Aminobenzamide, an inhibitor of poly(ADP-ribose) polymerse, but not the antioxidant N,N'-diphenylphenylenediamine, prevented the killing of Chinese hamster ovary cells by H2O2 and maintained the contents of NAD and ATP. The results of this study indicate that a moderate activation of the synthesis of S-adenosylmethionine leads to ATP and NAD depletion and to a greater sensitivity to cell killing by oxidative stress.	en_US
dc.description.sponsorship	This work was supported by grants from the Fondo de Investigaciones Sanitarias, Dirección General de Investigación Científica y Técnica, the EC Science Program, the National Institutes of Health and Europharma. E. S. G. is a fellow of the Comunidad Autónoma de Madrid.	en_US
dc.format.extent	389899 bytes	-
dc.format.mimetype	application/pdf	-
dc.language.iso	eng	en_US
dc.publisher	Portland Press	en_US
dc.publisher	Biochemical Society	-
dc.rights	closedAccess	en_US
dc.title	Increased sensitivity to oxidative injury in chinese hamster ovary cells stably transfected with rat liver S-adenosylmethionine synthetase cDNA	en_US
dc.type	artículo	en_US
dc.description.peerreviewed	Peer reviewed	en_US
dc.relation.publisherversion	http://www.biochemj.org/bj/319/0767/bj3190767.htm	en_US
dc.type.coar	http://purl.org/coar/resource_type/c_6501	es_ES
item.openairetype	artículo	-
item.grantfulltext	none	-
item.cerifentitytype	Publications	-
item.openairecristype	http://purl.org/coar/resource_type/c_18cf	-
item.fulltext	No Fulltext	-
item.languageiso639-1	en	-
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