Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/23814
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Martín-Sanz, Paloma | - |
dc.contributor.author | Cascales, Maria | - |
dc.contributor.author | Boscá, Lisardo | - |
dc.date.accessioned | 2010-05-03T08:24:13Z | - |
dc.date.available | 2010-05-03T08:24:13Z | - |
dc.date.issued | 1992-01-15 | - |
dc.identifier.citation | Biochemical Journal 281(2): 457-463 (1992) | en_US |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | http://hdl.handle.net/10261/23814 | - |
dc.description | 7 pages, 6 figures, 1 table. | en_US |
dc.description.abstract | Foetal and adult liver 6-phosphofructo-2-kinase (PFK-2) were purified by identical protocols. The native molecular masses of both enzymes were determined by gel filtration and were 89.1 and 100.0 kDa respectively. No differences were found in SDS/PAGE in 10%-acrylamide gel (55 kDa per subunit). The kinetic properties displayed by both enzymes were similar, except for the sensitivity to inhibition by sn-glycerol 3-phosphate. Foetal PFK-2 was a good substrate for phosphorylation by cyclic AMP-dependent protein kinase and protein kinase C, whereas the adult enzyme was phosphorylated only by cyclic AMP-dependent protein kinase. However, the phosphorylation affected only the kinetic properties of the adult enzyme, suggesting the presence in both enzymes of different sites of phosphorylation by cyclic AMP-dependent protein kinase. These differences in primary structure were consistent with the distinct chromatographic profiles of the phosphopeptides after digestion of the protein with CNBr. Western-blot analysis with antibodies specific for the N-terminal region of the liver-type PFK-2 poorly recognized the foetal enzyme, suggesting that both enzymes differ at least in the N-terminal sequence. | en_US |
dc.description.sponsorship | This work was supported by grant PM88-0025 from 'Comisión Interministerial de Ciencia y Tecnología, Spain'. | en_US |
dc.format.extent | 1488002 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Portland Press | en_US |
dc.rights | closedAccess | en_US |
dc.title | Characterization of 6-phosphofructo-2-kinase from foetal-rat liver | en_US |
dc.type | artículo | en_US |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1130707/pdf/biochemj00143-0160.pdf | en_US |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.fulltext | No Fulltext | - |
item.openairetype | artículo | - |
Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
336
checked on 14-abr-2024
Download(s)
99
checked on 14-abr-2024
Google ScholarTM
Check
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.