Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/237325
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dc.contributor.authorBleriot, Inés-
dc.contributor.authorTrastoy, Rocío-
dc.contributor.authorBlasco, Lucía-
dc.contributor.authorFernández-Cuenca, Felipe-
dc.contributor.authorAmbroa, Antón-
dc.contributor.authorFernández-García, Laura-
dc.contributor.authorPérez-Nadales, Elena-
dc.contributor.authorTorre-Cisneros, Julián-
dc.contributor.authorOteo, Jesús-
dc.contributor.authorNavarro, Ferrán-
dc.contributor.authorMiró, Elisenda-
dc.contributor.authorPascual, Álvaro-
dc.contributor.authorBou, Germán-
dc.contributor.authorMartínez-Martínez, Luis-
dc.contributor.authorTomás, María-
dc.date.accessioned2021-04-08T13:10:23Z-
dc.date.available2021-04-08T13:10:23Z-
dc.date.issued2020-04-29-
dc.identifierdoi: 10.1099/mgen.0.000369-
dc.identifiere-issn: 2057-5858-
dc.identifier.citationMicrobial Genomics 6 (2020)-
dc.identifier.urihttp://hdl.handle.net/10261/237325-
dc.description.abstractKlebsiella pneumoniae is the clinically most important species within the genus Klebsiella and, as a result of the continuous emergence of multi-drug resistant (MDR) strains, the cause of severe nosocomial infections. The decline in the effectiveness of antibiotic treatments for infections caused by MDR bacteria has generated particular interest in the study of bacteriophages. In this study, we characterized a total of 40 temperate bacteriophages (prophages) with a genome range of 11.454–84.199 kb, predicted from 16 carbapenemase-producing clinical strains of K. pneumoniae belonging to different sequence types, previously identified by multilocus sequence typing. These prophages were grouped into the three families in the order Caudovirales (27 prophages belonging to the family Myoviridae, 10 prophages belonging to the family Siphoviridae and 3 prophages belonging to the family Podoviridae). Genomic comparison of the 40 prophage genomes led to the identification of four prophages isolated from different strains and of genome sizes of around 33.3, 36.1, 39.6 and 42.6 kb. These prophages showed sequence similarities (query cover >90 %, identity >99.9 %) with international Microbe Versus Phage (MVP) (http://mvp.medgenius.info/home) clusters 4762, 4901, 3499 and 4280, respectively. Phylogenetic analysis revealed the evolutionary proximity among the members of the four groups of the most frequently identified prophages in the bacterial genomes studied (33.3, 36.1, 39.6 and 42.6 kb), with bootstrap values of 100 %. This allowed the prophages to be classified into three clusters: A, B and C. Interestingly, these temperate bacteriophages did not infect the highest number of strains as indicated by a host-range assay, these results could be explained by the development of superinfection exclusion mechanisms. In addition, bioinformatic analysis of the 40 identified prophages revealed the presence of 2363 proteins. In total, 59.7 % of the proteins identified had a predicted function, mainly involving viral structure, transcription, replication and regulation (lysogenic/lysis). Interestingly, some proteins had putative functions associated with bacterial virulence (toxin expression and efflux pump regulators), phage defence profiles such as toxin–antitoxin modules, an anti-CRISPR/Cas9 protein, TerB protein (from terZABCDE operon) and methyltransferase proteins-
dc.description.sponsorshipThis study was funded by grant PI16/01163 awarded to M.T. within the State Plan for R+D+I 2013–2016 (National Plan for Scientific Research, Technological Development and Innovation 2008–2011), and co-financed by the ISCIII-Deputy General Directorate for Evaluation and Promotion of Research – European Regional Development Fund ‘A Way of Making Europe’ and Instituto de Salud Carlos III FEDER, Spanish Network for the Research in Infectious Diseases (REIPI) (RD16/0016/0001, RD16/0016/0006 and RD16/0016/0008) and by the Study Group on Mechanisms of Action and Resistance to Antimicrobials (GEMARA) (Spanish Society of Infectious Diseases and Clinical Microbiology – SEIMC; http://www.seimc.org/). R.T. and L.F.-G. were financially supported by grants from the SEIMC and the Deputacion Provincial da Coruña (Xunta de Galicia), respectively-
dc.languageeng-
dc.publisherMicrobiology Society-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.subjectKlebsiella pneumoniae-
dc.subjectProphages-
dc.subjectBioinformatics-
dc.subjectGenomic analysis-
dc.subjectComparative genomics-
dc.subjectPhylogeny-
dc.titleGenomic analysis of 40 prophages located in the genomes of 16 carbapenemase-producing clinical strains of Klebsiella pneumoniae-
dc.typeartículo-
dc.relation.publisherversionhttp://dx.doi.org/10.1099/mgen.0.000369-
dc.date.updated2021-04-08T13:10:23Z-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderEuropean Commission-
dc.contributor.funderXunta de Galicia-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004587es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100010801es_ES
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.openairetypeartículo-
item.cerifentitytypePublications-
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