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Título

Carotenoid production in Lactobacillus plantarum

AutorGarrido Fernández, J. ; Maldonado-Barragán, Antonio ; Caballero-Guerrero, Belén ; Hornero-Méndez, Dámaso ; Ruiz-Barba, José Luis
Palabras claveLactobacillus plantarum
Carotenoid
Diaponeurosporene
Dehydrosqualene
Food fermentations
Fecha de publicación10-ene-2010
EditorElsevier
CitaciónInternational Journal of Food Microbiology 140(1): 34–39 (2010)
ResumenEighteen strains of Lactobacillus plantarum from different origins were screened for carotenoid production, as many of them exhibited a deep yellow pigmentation when cultured as isolated colonies on MRS-agar plates. We found that most of them produced significative amounts of the yellow C30 carotenoid 4,4'- diaponeurosporene in the range 1.8 to 54 mg/kg of dry cell weight. Although some of the strains produced just trace amounts of this carotenoid, PCR studies showed that all of them harbored the genes crtM and crtN which, inferred from homology, had been predicted in the three L. plantarum complete genome sequences currently available. Our results suggest the full functionality of a C30 carotenoid biosynthesis pathway in this species, driven by the operon crtNM. DNA sequencing of the entire crtNM operon in the maximum carotenoidproducing strain found in this study, i.e. L. plantarum CECT7531, was accomplished. Genes crtM and crtN were annotated as dehydrosqualene synthase and dehydrosqualene desaturase, respectively, in this strain
Versión del editorhttp://dx.doi.org/10.1016/j.ijfoodmicro.2010.02.015
URIhttp://hdl.handle.net/10261/23682
DOI10.1016/j.ijfoodmicro.2010.02.015
ISSN0168-1605
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