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dc.contributor.authorMercado-Blanco, Jesús-
dc.contributor.authorRodríguez-Jurado, Dolores-
dc.contributor.authorPérez-Artés, Encarnación-
dc.contributor.authorJiménez-Díaz, Rafael M.-
dc.date.accessioned2010-04-28T10:26:53Z-
dc.date.available2010-04-28T10:26:53Z-
dc.date.issued2002-01-
dc.identifier.citationEuropean Journal of Plant Pathology (2002), 108: 1–13en_US
dc.identifier.issn0929-1873 (Print)-
dc.identifier.issn1573-8469 (Online)-
dc.identifier.urihttp://hdl.handle.net/10261/23654-
dc.description13 pages, 6 figuresen_US
dc.description.abstractSpread of Verticillium wilt into newly established olive orchards in Andaluc´ıa, southern Spain, has caused concern in the olive industry in the region. This spread may result from use of Verticillium dahliae-infected planting material, which can extend distribution of the highly virulent, defoliating (D) pathotype of V. dahliae to newareas. In this study, a molecular diagnostic method for the early in planta detection of D V. dahliae was developed, aimed especially at nursery-produced olive plants. For this purpose, new primers for nested PCR were designed by sequencing a 992-bp RAPDmarker of theDpathotype. The use of the specific primers and different nested-PCR protocols allowed the detection of V. dahliae pathotype D DNA in infected root and stem tissues of young olive plants. Detection of the pathogen was effective from the very earliest moments following inoculation of olive plants with a V. dahliae pathotype D conidia suspension as well as in inoculated, though symptomless, plants.en_US
dc.description.sponsorshipGrants 1FD97-0763-CO3-01 from Comision Interministerial de Ciencia y Tecnología (CICYT) of Spain, and European Commission (Framework Programme 5; project reference number QLRT-1999-1523).en_US
dc.format.extent157338 bytes-
dc.format.mimetypeapplication/pdf-
dc.publisherSpringeren_US
dc.rightsclosedAccessen_US
dc.subjectMolecular diagnosisen_US
dc.subjectOlea europaeaen_US
dc.subjectPathogenen_US
dc.subjectFree certification programsen_US
dc.subjectVerticillium wilten_US
dc.titleDetection of the defoliating pathotype of Verticillium dahliae in infected olive plants by nested PCRen_US
dc.typeArtículoen_US
dc.identifier.doi10.1023/A:1013994827836-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1023/A:1013994827836en_US
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