Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/236508
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candida albicans interface |
Autor: | Díez-Orejas, Rosalía; Casarrubios, Laura; Feito, María José CSIC ORCID; Rojo, José María CSIC ORCID CVN ; Vallet-Regí, María; Arcos, Daniel; Portolés, María Teresa | Palabras clave: | Mesoporous nanospheres Macrophage Candida albicans Immune response Nanomaterial Phagocytosis |
Fecha de publicación: | may-2021 | Editor: | Elsevier | Citación: | International Immunopharmacology 94: 107457 (2021) | Resumen: | The use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects of the drug but, the uptake of these nanocarriers could induce adverse effects on cells and tissues after their incorporation. Macrophages play a central role in host defense and are responsible for in vivo nanoparticle trafficking. Assessment of their defense capacity against pathogenic micro-organisms after nanoparticle uptake, is necessary to prevent infections associated with nanoparticle therapies. In this study, the effects of hollow mesoporous SiO2-CaO nanospheres labeled with fluorescein isothiocyanate (FITC-NanoMBGs) on the function of peritoneal macrophages was assessed by measuring their ability to phagocytize Candida albicans expressing a red fluorescent protein. Two macrophage/fungus ratios (MOI 1 and MOI 5) were used and two experimental strategies were carried out: a) pretreatment of macrophages with FITC-NanoMBGs and subsequent fungal infection; b) competition assays after simultaneous addition of fungus and nanospheres. Macrophage pro-inflammatory phenotype markers (CD80 expression and interleukin 6 secretion) were also evaluated. Significant decreases of CD80+ macrophage percentage and interleukin 6 secretion were observed after 30 min, indicating that the simultaneous incorporation of NanoMBG and fungus favors the macrophage non-inflammatory phenotype. The present study evidences that the uptake of these nanospheres in all the studied conditions does not alter the macrophage function. Moreover, intracellular FITC-NanoMBGs induce a transitory increase of the fungal phagocytosis by macrophages at MOI 1 and after a short time of interaction. In the competition assays, as the intracellular fungus quantity increased, the intracellular FITC-NanoMBG content decreased in a MOI- and time-dependent manner. These results have confirmed that macrophages clearly distinguish between inert material and the live yeast in a dynamic intracellular incorporation. Furthermore, macrophage phagocytosis is a critical determinant to know their functional state and a valuable parameter to study the nanomaterial / macrophages / Candida albicans interface. | Descripción: | 35 p.-6 fig.-2 tab. | Versión del editor: | https://doi.org/10.1016/j.intimp.2021.107457 | URI: | http://hdl.handle.net/10261/236508 | DOI: | 10.1016/j.intimp.2021.107457 | ISSN: | 1567-5769 |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
IntImmunopharmacol 2021 94 107457 JM Rojo preprint.pdf | Preprint | 1,17 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
7
checked on 20-mar-2024
WEB OF SCIENCETM
Citations
7
checked on 28-feb-2024
Page view(s)
89
checked on 29-mar-2024
Download(s)
99
checked on 29-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.