Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/235280
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK

AutorBleriot, Inés; Blasco, Lucía; Delgado-Valverde, Mercedes CSIC ORCID; Gual-de-Torrella, Ana CSIC ORCID; Ambroa, Antón; Fernández-García, Laura; López, María; Oteo-Iglesias, Jesús; Wood, Thomas K.; Pascual, Álvaro CSIC ORCID; Bou, Germán; Fernández-Cuenca, Felipe CSIC ORCID CVN; Tomás, María
Palabras claveTolerance
Persistence
Cross-resistance
Toxin-antitoxin system
PemI/PemK
Klebsiella pneumoniae
Fecha de publicación2-sep-2020
EditorMultidisciplinary Digital Publishing Institute
CitaciónToxins 12(9): 566 (2020)
ResumenAlthough the failure of antibiotic treatment is normally attributed to resistance, tolerance and persistence display a significant role in the lack of response to antibiotics. Due to the fact that several nosocomial pathogens show a high level of tolerance and/or resistance to chlorhexidine, in this study we analyzed the molecular mechanisms associated with chlorhexidine adaptation in two clinical strains of Klebsiella pneumoniae by phenotypic and transcriptomic studies. These two strains belong to ST258-KPC3 (high-risk clone carrying β-lactamase KPC3) and ST846-OXA48 (low-risk clone carrying β-lactamase OXA48). Our results showed that the K. pneumoniae ST258-KPC3CA and ST846-OXA48CA strains exhibited a different behavior under chlorhexidine (CHLX) pressure, adapting to this biocide through resistance and tolerance mechanisms, respectively. Furthermore, the appearance of cross-resistance to colistin was observed in the ST846-OXA48CA strain (tolerant to CHLX), using the broth microdilution method. Interestingly, this ST846-OXA48CA isolate contained a plasmid that encodes a novel type II toxin/antitoxin (TA) system, PemI/PemK. We characterized this PemI/PemK TA system by cloning both genes into the IPTG-inducible pCA24N plasmid, and found their role in persistence and biofilm formation. Accordingly, the ST846-OXA48CA strain showed a persistence biphasic curve in the presence of a chlorhexidine-imipenem combination, and these results were confirmed by the enzymatic assay (WST-1).
DescripciónThis article belongs to the Special Issue Toxin-Antitoxin Systems in Pathogenic Bacteria.
Versión del editorhttp://doi.org/10.3390/toxins12090566
URIhttp://hdl.handle.net/10261/235280
DOI10.3390/toxins12090566
Identificadoresdoi: 10.3390/toxins12090566
e-issn: 2072-6651
Aparece en las colecciones: (IBIS) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
PemIK.pdf1,72 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

10
checked on 11-mar-2024

SCOPUSTM   
Citations

15
checked on 12-mar-2024

WEB OF SCIENCETM
Citations

11
checked on 27-feb-2024

Page view(s)

75
checked on 18-mar-2024

Download(s)

178
checked on 18-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.