English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/23357
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Detection of the defoliating and nondefoliating pathotypes of Verticillium dahliae in artificial and natural soils by nested PCR

AutorPérez-Artés, Encarnación ; Mercado-Blanco, Jesús ; Ruz-Carrillo, Ana Rosa; Rodríguez-Jurado, Dolores; Jiménez-Díaz, Rafael M.
Palabras claveCotton
Infested-soil
Molecular detection
Olive
Verticillium wilt disease
Fecha de publicaciónene-2005
EditorSpringer
CitaciónPlant and Soil (2005) 268: 349–356
ResumenIn Spain, Verticillium wilt, caused by Verticillium dahliae, is the most important disease of cotton and olive. Isolates of V. dahliae infecting these crops can be classified into highly virulent, defoliating (D), and mildly virulent, nondefoliating (ND), pathotypes. Infested soil is the primary source of inoculum for Verticillium wilt epidemics in cotton and olive, and severity of disease relates to the prevailing V. dahliae pathotype. In this work we have adapted the use of previously developed primer pairs specific for D and ND V. dahliae for the detection of these pathotypes by nested PCR in artificial and natural soils. Success in the detection procedure depends upon efficiency in extracting PCR-quality DNA from soil samples. We developed an efficient DNA extraction method from microsclerotia infesting the soil that includes the use of acid washed sand during the grinding process and skimmed milk to avoid co-purification of Taq-polymerase inhibitors with DNA. The specific nested-PCR procedure effectively detected 10 or more microsclerotia per gram of soil. The detection procedure has proven efficient when used with a naturally infested soil, thus demonstrating usefullness of the diagnostic method for rapid and accurate assessment of soil contamination by V. dahliae pathotypes.
Descripción8 pages; 5 figures
Versión del editorhttp://dx.doi.org/10.1007/s11104-004-0378-1
URIhttp://hdl.handle.net/10261/23357
DOI10.1007/s11104-004-0378-1
ISSN0032-079X (print version)
1573-5036 (Online)
Aparece en las colecciones: (IAS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Digital CSIC. Carta.pdf153,65 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.