Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/233171
COMPARTIR / EXPORTAR:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva

AutorSaha, Arpita; Ben Halima, Hamdi; Saini, Abhishek; Gallardo González, Juan; Zine, Nadia; Viñas, Clara CSIC ORCID; Elaissari, Abdelhamid; Errachid, Abdelhamid; Teixidor, Francesc CSIC ORCID
Palabras claveimmobilization of antibodies
IL-10
magnetic nanoparticles
pre-concentration of antigens
saliva matrix
TNF-α
Fecha de publicaciónsep-2020
EditorMolecular Diversity Preservation International
CitaciónMolecules 25(17): 3968 (2020)
ResumenMagnetic nanoparticles (MNPs) were synthesized using the colloidal co-precipitation method and further coated with silica using the Stöber process. These were functionalized with carboxylic and amine functionalities for further covalent immobilization of antibodies on these MNPs. The procedure for covalent immobilization of antibodies on MNPs was developed using 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The evaluation of the efficiency of the coupling reaction was carried out by UV-vis spectrophotometry. The developed antibodies coupled to MNPs were tested for the pre-concentration of two biomarkers tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Both biomarkers were assessed in the matrix based on phosphate-buffered saline solution (PBS) and artificial saliva (AS) to carry out the demonstration of the format assay. Supernatants were used to determine the number of free biomarkers for both studies. Reduction of the nonspecific saliva protein adsorption on the surface of the complex antibodies-MNPs to levels low enough to allow the detection of biomarkers in complex media has been achieved
Versión del editorhttp://dx.doi.org/10.3390/molecules25173968
URIhttp://hdl.handle.net/10261/233171
E-ISSN1420-3049
Aparece en las colecciones: (ICMAB) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
Saha_Molecules_2020_editorial.pdf3,41 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

Page view(s)

58
checked on 28-mar-2024

Download(s)

73
checked on 28-mar-2024

Google ScholarTM

Check


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.