Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/233170
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Immobilization of BMP-2 and VEGF within Multilayered Polydopamine-Coated Scaffolds and the Resulting Osteogenic and Angiogenic Synergy of Co-Cultured Human Mesenchymal Stem Cells and Human Endothelial Progenitor Cells |
Autor: | Godoy Gallardo, María; Portolés Gil, Núria; López Periago, Ana M. CSIC ORCID ; Domingo Pascual, M. Concepción; Hosta Rigau, Leticia | Palabras clave: | Angiogenesis Bone tissue engineering Co-delivery Growth factors Multilayers Osteogenesis Ppolydopamine Scaffolds |
Fecha de publicación: | sep-2020 | Editor: | Molecular Diversity Preservation International | Citación: | International Journal of Molecular Sciences 21(17): 6418 (2020) | Resumen: | We have previously reported the fabrication of a polycaprolactone and hydroxyapatite composite scaffold incorporating growth factors to be used for bone regeneration. Two growth factors were incorporated employing a multilayered coating based on polydopamine (PDA). In particular, Bone morphogenetic protein-2 (BMP-2) was bound onto the inner PDA layer while vascular endothelial growth factor (VEGF) was immobilized onto the outer one. Herein, the in vitro release of both growth factors is evaluated. A fastest VEGF delivery followed by a slow and more sustained release of BMP-2 was demonstrated, thus fitting the needs for bone tissue engineering applications. Due to the relevance of the crosstalk between bone-promoting and vessel-forming cells during bone healing, the functionalized scaffolds are further assessed on a co-culture setup of human mesenchymal stem cells and human endothelial progenitor cells. Osteogenic and angiogenic gene expression analysis indicates a synergistic effect between the growth factor-loaded scaffolds and the co-culture conditions. Taken together, these results indicate that the developed scaffolds hold great potential as an efficient platform for bone-tissue applications. | Versión del editor: | http://dx.doi.org/10.3390/ijms21176418 | URI: | http://hdl.handle.net/10261/233170 | E-ISSN: | 1422-0067 |
Aparece en las colecciones: | (ICMAB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Godoy_IntJMolSci_2020_editorial.pdf | 4,56 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
56
checked on 18-abr-2024
Download(s)
125
checked on 18-abr-2024
Google ScholarTM
Check
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.