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Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice

AuthorsSoto, Manuel ; Ramírez, Laura ; Solana, José Carlos; Cook, Emma C. L.; Hernández-García, Elena; Requena, José María; Iborra, Salvador
Issue Date12-Feb-2021
PublisherMultidisciplinary Digital Publishing Institute
CitationMicroorganisms 9 (2): 363 (2021)
Abstract<i>Leishmania amazonensis</i> parasites are etiological agents of cutaneous leishmaniasis in the New World. BALB/c mice are highly susceptible to <i>L. amazonensis</i> challenge due to their inability to mount parasite-dependent IFN-γ-mediated responses. Here, we analyzed the capacity of a single administration of the <i>LiΔHSP70-II</i> genetically-modified attenuated <i>L. infantum</i> line in preventing cutaneous leishmaniasis in mice challenged with <i>L. amazonensis</i> virulent parasites. In previous studies, this live attenuated vaccine has demonstrated to induce long-protection against murine leishmaniasis due to Old World <i>Leishmania</i> species. Vaccinated mice showed a reduction in the disease evolution due to <i>L. amazonensis</i> challenge, namely reduction in cutaneous lesions and parasite burdens. In contrast to control animals, after the challenge, protected mice showed anti-<i>Leishmania</i> IgG2a circulating antibodies accompanied to the induction of <i>Leishmania</i>-driven specific IFN-γ systemic response. An analysis performed in the lymph node draining the site of infection revealed an increase of the parasite-specific IFN-ϒ production by CD4<sup>+</sup> and CD8<sup>+</sup> T cells and a decrease in the secretion of IL-10 against leishmanial antigens. Since the immunity caused by the inoculation of this live vaccine generates protection against different forms of murine leishmaniasis, we postulate <i>LiΔHSP70-II</i> as a candidate for the development of human vaccines.
Identifiersdoi: 10.3390/microorganisms9020363
Appears in Collections:Colección MDPI
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