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Título

Crystallization of the pneumococcal autolysin LytC: in-house phasing using novel lanthanide complexes

AutorPérez-Dorado, Inmaculada ; Sanles, Reyes ; González, Ana ; García, Pedro ; García, José Luis ; Martínez-Ripoll, Martín ; Hermoso, Juan A.
Palabras claveAutolysins
LytC
Gd-HPDO3A
Fecha de publicaciónabr-2010
EditorInternational Union of Crystallography
CitaciónActa Crystallographica Section F 66(4): 448-451 (2010)
ResumenLytC, one of the major autolysins from the human pathogen Streptococcus pneumoniae, has been crystallized as needles by the hanging-drop technique using 10%(w/v) PEG 3350 as precipitant and 10 mM HEPES pH 7.5. LytC crystals were quickly soaked in mother liquor containing 2 mM of the complex Gd-HPDO3A to produce derivatized crystals (LytC(Gd-HPDO3A)). Both native LytC and isomorphous LytC(Gd-HPDO3A) crystals were flash-cooled in a nitrogen flow at 120 K prior to X-ray data collection using an in-house Enraf-Nonius rotating-anode generator (lambda = 1.5418 A) and a MAR345 imaging-plate detector. In both cases, good-quality diffraction patterns were obtained at high resolution. LytC(Gd-HPDO3A) crystals allowed the collection of a SAD X-ray data set to 2.6 A resolution indexed in terms of a P2(1) monoclinic unit cell with parameters a = 59.37, b = 67.16, c = 78.85 A, beta = 105.69 degrees . The anomalous Patterson map allowed the identification of one heavy-atom binding site, which was sufficient for the calculation of an interpretable anomalous map at 2.6 A resolution.
Descripción4 pages, 3 figures, 1 table.-- PMID: 20383019 [PubMed]
Versión del editorhttp://dx.doi.org/10.1107/S1744309110006081
URIhttp://hdl.handle.net/10261/23090
DOI10.1107/S174430911000608
ISSN1744-3091
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