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DAMPs and NLRP3 Inflammasome Response in Fatty Liver Grafts Preservation in Different Preservation Solutions

AuthorsBardallo, R. G.; Teixeira-Silva, R. G.; Panisello-Roselló, Arnau; Folch-Puy, Emma CSIC ORCID ; Roselló-Catafau, Joan CSIC ORCID; Carbonell, Teresa
Liver preservation
Issue DateApr-2020
PublisherJohn Wiley & Sons
CitationAmerican Journal of Transplantation 20(S3) (2020)
AbstractPurpose: Preservation solutions (UW, HTK and IGL-1) are widely used in clinical liver transplantation. It is well known that the composition of the preservation solution determines the outcome of the transplanted liver. It has been demonstrated that PEG35 is a key factor in the IGL-1 preservation solution. Therefore, a modified IGL-1 solution (with increased PEG concentration) was compared to HTK and UW preservation solutions. The aim of this communication is to investigate the effects of the oncotic agent PEG 35 on NLRP3 inflammasome activity and redox state during static preservation. Methods: Fatty livers from male Zücker obese rats were preserved for 24 h at 4ºC in the studied solutions. After organ recovery and rinsing liver grafts with Ringer Lactate solution, liver injury was measured as AST/ALT and GLDH; redox state as GSH/GSSG, GST, GR, GPx, 4HNE; and inflammasome and precursors measured as NLRP3, HMGB1, and GRP78. Results: Increases in the reducing redox capability of the cell are associated with decreased inflammasome activation and liver damage. Conclusions: Data reported here show that the solution with increased content of PEG35 (Modified IGL-1) well correlated with a better protection through a significant prevention of inflammation in front of UW and HTK.
DescriptionResumen del trabajo presentado en el American Transplant Congress 2020, originalmente programado para tener lugar en Filadelfia (Estados Unidos), se convirtió en un Congreso Virtual debido a COVID-19, celebrado del 30 de mayo al 3 de junio de 2020
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Appears in Collections:(IIBB) Comunicaciones congresos

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